In vitro growth inhibition of Aspergillus flavus, a major aflatoxin producing fungus, using Thymus eriocalyx essential oil
Journal Title: Scientia Agriculturae - Year 2015, Vol 10, Issue 3
Abstract
In this study the effect of Thymus eriocalyx essential oil on the growth of Aspergillus flavus, a major producer of aflatoxin, was evaluated in vitro. The assessment was done by conducting an experimental completely randomized design comprising of six treatments and four replications. The treatments were four concentrations of the thyme essential oil (0.02, 0.06, 0.1 and 0.14 mg/ml) and two controls (Dimethyl sulfoxide and fungicide solutions). Each treatment was applied to the fungus by placing a paper disc, impregnated with each treatment, in the center of a plate of PDA medium inoculated with spore suspension of the fungus. Measuring the average diameter of A. flavus growth inhibition zone around the impregnated paper discs indicated that compared to the negative control, all concentrations of thyme essential oil significantly inhibited the mycelial growth of the fungus. Three concentrations of the thyme essence used (0.06, 0.1 and 0.14 mg/ml) could significantly control the growth of the fungus more than did the fungicide treatment. Considering these results it seems that Thymus eriocalyx essential oil is potentially a natural alternative for controlling A. flavus mold.
Authors and Affiliations
Jafar Nikan, Sahar Ghamari
Keywords
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- EP ID EP148197
- DOI 10.15192/PSCP.SA.2015.10.3.110114
- Views 115
- Downloads 0
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