Leuconostoc mesenteroides NRRL B-1149 as probiotic and its dextran with anticancer properties
Journal Title: Journal of BioScience and Biotechnology - Year 2014, Vol 3, Issue 1
Abstract
Leuconostoc mesenteroides NRRL B-1149 was evaluated for its probiotic properties. It displayed in vitro cell surface traits in terms of hydrophobicity, autoaggregation property and cell adhesive capacity on HeLa, murine macrophage and HT29 cell lines, which revealed its capability to adhere and colonize the intestine. The tolerance to various biological barriers such as lysozyme (100 μg/ml), gastric juice (pH 3.0) and bile salts (0.5%, w/v) with 92%, 61.6% and 219% growth, respectively, confirmed its ability to survive in extreme conditions of digestive tract. It showed bile salts hydrolase activity signifying its ability to deconjugate bile salts. The whole cells of Leuconostoc mesenteroides B-1149 showed β-galactosidase activity with 94 Miller units displaying its importance in lactose utilization. The effect of dextran from Leuconostoc mesenteroides B-1149 having α-(1,6) linear glycosidic linkages and α-(1,3) branching was evaluated against cervical cancer (HeLa) and colon cancer (HT29) cell lines. The dextran from L. mesenteroides B-1149 (100 μg/ml) showed 25% inhibition of HeLa cell lines and 1000 μg/ml dextran displayed 40% inhibition of HT29 cell lines. The effect of dextran on murine monocyte-macrophage line (J774A.1) showed increase in growth of macrophages confirming its biocompatible nature.
Authors and Affiliations
Rishikesh Shukla, Ilia Iliev, Arun Goyal
Evaluation of dietary supplementation of B vitamins and HMBi on fermentation kinetics, ruminal or post-ruminal diet digestibility using modified in vitro techniques
The effects of dietary supplementation of HMBi, alone or in combination with B vitamins were investigated on fermentation characteristics and in vitro ruminal and post-ruminal digestion using three modified techniques. A...
Changes in composition and structure of breeding bird communities due to successive changes in ecosystems
Successive changes in ecosystems are due to natural and/or anthropogenic influences. These changes influence species richness and structure of phyto- and zoocenoses. Birds represent a good indicator for changes of...
Genetic diversity among salt-tolerant rice (Oryza sativa L.) landraces cultivated in the coastal districts of Bangladesh
The aim of this study was to determine genetic diversity and relationships among the salt-tolerant rice landraces cultivated in the coastal districts of Bangladesh. DNA extracted from seedlings of nine salt-toleran...
Enhanced heterologous gene expression in diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha
The expression of bacterial gusA gene encoding for E. coli enzyme β-glucuronidase in haploid and diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha was studied. Haploid strains were transformed wi...
Study of root distribution of Macaranga tanarius (L.) Müll. Arg. (Parasol leaf tree) on East-West highway slope, Malaysia
Vegetation influences the stability of slope by both hydrological events and mechanical reinforcement of the soil. The magnitude of such effects is subject to the root system growth, which in turn is a purpose of the gen...