MICROBIAL CONVERSION OF SUGAR CANE PHYTOSTEROLS BY FUSARIUM SOLANI
Journal Title: Asian J of Pharm Sci & Tech - Year 2014, Vol 4, Issue 1
Abstract
ABSTRACT Air dried press cake of sugar cane (5 Kg) yielded on extraction with cold acetone 500 g of waxy residue which gave on saponification 150gm. Un-saponifiable matter. Gas chromatography and mass spectroscopic analysis of the present sterols revealed that the phytosterol mixture consists of - sit sterol (46.4%), stigma sterol (21.4%) and camp sterol (27.9 %).This sterol mixture was subjected to the activities of some selected locally isolated fungal strains. Fusarium solani was the most potent organism to produce 4-androstenedione (AD) and 1, 4 androstadiene 3, 17 dione (ADD). The maximum yield of ADD (34.6%) was obtained in a medium containing (g/l) 0.15 MgSO4. 7H2O, 0.188, KH2PO4, 0.38, (NH4)2 HPO4, 0.1 , CaCl2. 2H2O, 0.29 , NaCl, 0.001 FeSO4 and 0.8 mg 8-hydroxyquinoline, pH 7.1at transformation time 48 hr, 30°C . Key words: Sugar cane phytosterols, AD, ADD, Fusarium solani.
Authors and Affiliations
Abd-Alla A
Keywords
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