Phytochemical and therapeutic evaluation of leaf and in vitro derived callus and shoot of Solanum trilobatum
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 6
Abstract
This study focuses on the phytochemical properties and the anti-hepatocarcinogenic effects of the leaf and in vitro-derived callus and shoot extracts of Solanum trilobatum. In the leaf, callus and shoot, the presence of sugar, proteins, alkaloids, flavonoids, saponins, tananins, cardiacglycoside, terpenoid and lipids was established by preliminary phytochemical screening. Surface-sterilized explants (0.5-1.0 cm) were placed on the MS basal medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (0.45, 2.26, 4.52, 11.31 and 12.56mM), naphthylacetic acid (NAA; 0.54, 1.34, 2.69, 5.37, 13.43 and 26.85mM) and 6-benzylaminopurine (BA; 0.44, 1.11, 2.22, 4.44, 8.88 and 13.32mM) for callus induction. Explants from node and callus culture were inoculated on the MS basal medium supplemented with varying concentrations of BA (0.44-22.20 mM) and NAA (0.54-10.74mM) for shoot multiplication. Rats were divided into five groups and administered with diethyl nitrosamine (DEN) and DEN (200mg/kg bwt) intraperitoneally along with methanol leaf and in vitro-derived callus and shoot extracts (250mg/kg bwt) orally for 3 months. A significant deviation (P<0.05) in marker enzymes such as alanine transaminase, aspartate transaminase, lactate dehydrogenase and total bilirubin was found in rats administered with DEN. The liver tissue was used for the analysis of glutathione reductase, lipid peroxidation, glutathione peroxidase, glutathione S-transferase, superoxide dismutase and catalase. DEN administration caused a significant elevation in serum enzymes and total bilirubin. Moreover, antioxidant enzymes were drastically inhibited with significant reduction in glutathione and increased lipid per oxidation. Increased glutathione level and reduced lipid peroxidation were also evident in S. trilobatum-treated rats. However, crude S. trilobatum and in vitro-derived callus and shoot extracts offered better protection against free radical toxicity induced by DEN.
Authors and Affiliations
Priya Govindarajan , Chellaram Chinnachamy
Keywords
Related Articles
- EP ID EP105463
- DOI -
- Views 102
- Downloads 0
Antioxidant and antibacterial activities of extracts from Conyza bonariensis growing in Yemen
This study aims to examine the antioxidant and antibacterial activities and phenolic contents of Conyza bonariensis growing in Yemen. The whole plants of C. bonariensis were ultrasonically extracted by ethanol. The...
In vitro cytotoxic activity of Aesculus indica against breast adenocarcinoma cell line (MCF-7) and phytochemical analysis
Aesculus indica (Linn.) (Sapindaceae) is an ethanobotanically important plant specie traditionally used against rheumatism, skin and vein complaints. Cytotoxic potential of Aesculus indica crude leaf extract and its frac...
Combined use of borneol or menthol with labrasol promotes penetration of baicalin through rabbit cornea in vitro
The permeability of most drugs through the eyes is very limited, so finding safe and effective penetration enhancers is of high importance in current ophthalmology research. In this paper, we use a new approach tha...
Assessment of antinociceptive, antipyretic and antimicrobial activity of Piper cubeba L. essential oil in animal models
This study was designed to investigate the possible antiniciceptive, antipyretic and antimicrobial activities of the essential oil obtained from the fruits of Piper Cubeba (L.). To assess the antinociceptive and an...
Mutagenic breeding of Quambalaria cyanescens strain for the production of milk-clotting enzymes
A strain for producing rennet was screened from red kojic rice and identified as Quambalaria cyanescens. In the current work, various mutagenic treatments, such as UV, diethyl sulfate and their combinations were ap...