Prevalence of Malassezia speciesassociated with dandruff in South India- A study among mild to moderately affected individuals
Journal Title: International Journal of Advanced Biotechnology and Research. - Year 2016, Vol 7, Issue 1
Abstract
ABSTRACT Objective: Yeasts of the Malassezia genus have been extensively studied and correlated with various dermatological diseases such as pityriasisversicolor, seborrheic dermatitis (SD) & dandruff (SD/D), atopic dermatitis, psoriasis, and also less frequently with confluent and reticulated papillomatosis, onychomycosis, and transient acantholyticdermatosis. In general, Malassezia yeasts are lipid-dependent and commonly found in sebum-rich areas of the skin such as the scalp, trunk and face. The aim of this study was to determine the prevalence of Malassezia species among individuals who are suffering from mild to moderate dandruff in the southern part of India (Bengaluru region) and to identify these yeast species using the molecular technique to elucidate the distribution pattern. This study also attempted to test phospholipase production of isolated yeast to determine their pathogenic potential. . Methods: Scraped flake samples collected from 45 volunteers in Bengaluru region were further subjected to isolation of Malassezia species using agar media. Isolated Malassezia yeasts were further identified by PCRRFLP. Phospholipase production was done using semi-quantitative egg-yolk plate method. Results: Out of 50 Malassezia isolates, the most common species was M. furfur (64.7%) followed by M. restricta and M. slooffiae(14% each)and finally the least of them all were M. sympodialis and M. globosa (4% each). None out of 50 Malassezia strains showed clearly distinguishable precipitation zone for phospholipase activity upon 5 days incubation (Pz value after 5 days was 1). Conclusion - Incidence of Malassezia furfur was significantly higher than M. globosa, M. restricta, M. slooffiae, M. sympodialis and M. globosa. Much longer incubation time is required to check the capacity to produce distinguishable level of phospholipase enzymes by these Malassezia isolates.
Authors and Affiliations
K. Kavitha1,3 , P. Sekar2 , R. Pirabhakaran3 and T. Mukhopadhyay3
Keywords
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