QUALITATIVE STUDIES ON THE PRODUCTION OF STEVIOSIDE IN IN VITRO CALLUS CULTURE OF STEVIA REBAUDIANA BERTONI
Journal Title: Analele Stiintifice ale Universitatii Alexandru Ioan Cuza din Iasi, Serie noua, Sectiunea II a. Biologie vegetala - Year 2005, Vol 51, Issue 0
Abstract
Stevioside is a glycoside of the diterpene derivatives of steviol. Steviol glycosides are natural constituents of the plant Stevia rebaudiana Bertoni belonging to the Compositae family. The leaves of S. rebaudiana contain eight different steviol glycosides. The major constituent stevioside, is present in dry leaves to the tune of 5 to 10%. Stevioside has a sweetening potency of 250 to 300 times that of sucrose. The cultivation of Stevia rebaudiana by seeds is not adequate because of poor germination and random variation in the content of stevioside due to genetic variability in plants. Therefore, efforts were made to standardize the media requirements including the level of growth regulators to obtain optimum callus production which can then be used as the source material for extraction of stevioside. Leaf explants with slight nodal portion from in vitro raised plantlets inoculated on MS medium supplemented with 2.0 mg l-1 NAA and 0.3 mg l-1 BA gave early initiation (12 to 14 days) of callus as well as maximum amount of callus. The callus on subculturing on the same medium multiplied and recorded maximum growth. The presence of stevioside in callus was evaluated using Thin Layer Chromatography and this found to be positive.
Authors and Affiliations
R. JADEJA, M. TADHANI, S. REMA, L. PAREKH
Keywords
Related Articles
- EP ID EP145471
- DOI -
- Views 123
- Downloads 0
CLUSTER ANALYSIS AND VEGETATION MESO-XERIC ORDERING IN MORIŞCA VALLEY BASIN (BOTOŞANI COUNTY)
The aim of this paper is to classify, describe, and interpret the floristic structure of meso-xeric plant communities in the Morişca Valley, by eco-cenotic data numerical analysis, interlinked with environmental gradient...
EVALUATION OF CYTOTOXIC AND GENOTOXIC POTENTIAL OF THE FUNGICIDE RIDOMIL IN ALLIUM CEPA L.
The cytogenetic effects exerted by the systemic fungicide mefenoxam and copper hydroxide (the active ingredients of Ridomil Gold Plus 42,5 WP fungicide) were studied in root tips of Allium cepa L. A progressive concentra...
THE EFFECT OF HEAT STRESS ON HYPERHYDRICITY AND GUAIACOL PEROXIDASE ACTIVITY (GPOX) AT THE FOLIAR LAMINA OF SEDUM TELEPHIUM L. SSP. MAXIMUM (L.) KROCK. VITROPLANTLETS
Heat stress causes an array of physiological, biochemical and morphological changes in plants, affecting plant growth and development. In order to confirm the connection between oxidative stress in plants and hyperhydric...
REVISION OF THE ORCHIDACEAE FAMILY FROM THE HERBARIUM OF “ALEXANDRU IOAN CUZA” UNIVERSITY OF IAŞI (4TH NOTE)
This paper presents the last note about the genera of Orchidaceae family that are stored in Herbarium collection from “Alexandru Ioan Cuza” University of Iaşi [I]. The genera, Dactylorhiza and Orchis, have been revised f...
CONTRIBUTIONS TO THE HISTO-ANATOMICAL STUDY OF THE CALENDULA OFFICINALIS L. LEAVES TREATED WITH THIOPHANATE METHYL (TOPSIN M)
The study analyzes the histo-anatomical modifications of the Calendula officinalis leaf, caused by the treatment with thiophanate methyl, applied 3 times, in two different concentrations, of 0.1% and, respectively,...