Near-field electrospinning of a polymer/bioactive glass composite to fabricate 3D biomimetic structures
Journal Title: International Journal of Bioprinting - Year 2019, Vol 5, Issue 1
Abstract
Bioactive glasses have recently gained attention in tissue engineering and three-dimensional (3D) bioprinting because of their ability to enhance angiogenesis. Some challenges for developing biological tissues with bioactive glasses include incorporation of glass particles and achieving a 3D architecture mimicking natural tissues. In this study, we investigate the fabrication of scaffolds with a polymer/bioactive glass composite using near-field electrospinning (NFES). An overall controlled 3D scaffold with pores, containing random fibers, is created and aimed to provide superior cell proliferation. Highly angiogenic borate bioactive glass (13-93B3) in 20 wt.% is added to polycaprolactone (PCL) to fabricate scaffolds using the NFES technique. Scaffolds measuring 5 mm × 5 mm × 0.2 mm3 in overall dimensions were seeded with human adipose-derived mesenchymal stem cells to investigate the cell viability. The cell viability on PCL and PCL+glass scaffolds fabricated using NFES technique and 3D printing is compared and discussed. The results indicated higher cell proliferation on 3D biomimetic scaffolds fabricated by NFES technique.
Authors and Affiliations
Krishna C. R. Kolan, Jie Li, Sonya Roberts, Julie A. Semon, Jonghyun Park, Delbert E. Day, Ming C. Leu
Fabrication of biomimetic placental barrier structures within a microfluidic device utilizing two-photon polymerization
The placenta is a transient organ, essential for development and survival of the unborn fetus. It interfaces the body of the pregnant woman with the unborn child and secures transport of endogenous and exogenous substanc...
3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering
Three-dimensional (3D) printing of hydrogels is now an attractive area of research due to its capability to fabricate intricate, complex and highly customizable scaffold structures that can support cell adhesion and prom...
A novel 3D printing method for cell alignment and differentiation
The application of bioprinting allows precision deposition of biological materials for bioengineering applications. Here we propose a 2 stage methodology for bioprinting using a back pressure-driven, automated robotic di...
3D bioprinting for tissue engineering: Stem cells in hydrogels
Surgical limitations require alternative methods of repairing and replacing diseased and damaged tissue. Regenerative medicine is a growing area of research with engineered tissues already being used successfully in pati...
An nMgO containing scaffold: Antibacterial activity, degradation properties and cell responses
Bone repair failure caused by implant-related infections is a common and troublesome problem. In this study, an antibacterial scaffold was developed via selective laser sintering with incorporating nano magnesium oxide (...