NEW SECONDARY METABOLITE AND BIOACTIVITIES OF ASPHODELUS REFRACTUS
Journal Title: Indo American Journal of Pharmaceutical Sciences - Year 2018, Vol 5, Issue 5
Abstract
An investigation of the chemical constituents of the ethanolic extract of Asphodelus refractus herb, led to the isolation of new 1,3,4-trihydroxy-8-methoxy-2-methyl anthraquinone- Refractlin(2), together with four known Compounds 1,3,4, and 5 were identified as Chryseophanol, Luteolin, p- hydroxyphenethyl trans-ferulate and 7 hydroxy emodin, respectively. Their chemical structures have been deduced on the basis of 1D (1H and 13C), 2D (HMQC, and HMBC) NMR, UV, IR and ESI/MS spectral studies. The total extract and Ethyl acetate extract were evaluated for their cytotoxic activity against human Hepatocellular carcinoma (HepG-2), Colon carcinoma (HCT116), and Lung carcinoma (A-549) cell lines. The antioxidant activities were also evaluated using 1, 1-diphenyl-2- picrylhydrazyl free radical (DPPH), assay method. The measurement of polyphenolics by folin - ciocalteau reagent and flavonoid by NaNO2-AlCl3-NaOH indicated that 30 mg rutin and 52 mg gallic acid equivalents/1gram of powdered Asphodelus refractus herb, respectively. Keywords: Asphodelus, Refractlin , Antimicrobial, Antioxidant, Cytotoxicity.
Authors and Affiliations
Keywords
Related Articles
- EP ID EP292395
- DOI -
- Views 299
- Downloads 0
TOOTH CARVING SESSIONS AND PERCEPTION OF DENTAL STUDENTS’ REGARDING UNDERSTANDING OF TOOTH MORPHOLOGY
The study was aimed at study the perception of dental students at Baqai dental college regarding tooth carving sessions and how it is helpful in developing their understanding and skills of the subject of tooth morpholog...
ESTIMATION OF XIPAMIDE BY USING HPLC IN PURE AND PHARMACEUTICAL DOSAGE FORM
A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validated of Xipamide in its pure form as well as in tablet dosage form. Chromatography was carried out on Symme...
A NEW STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ERTUGLIFLOZIN AND SITAGLIPTIN IN BULK AND PHARMACEUTICAL DOSAGE FORM ITS VALIDATION AS PER ICH GUIDELINES
A new RP-HPLC method for the quantitative determination of Ertugliflozin and Sitagliptin was developed and validated as per ICH guidelines. The drugs were injected into Std Azilent column (150×4.6, 5 μm), maintained at a...
FOLLOW UP STUDY FOR THREE YEARS OF CONGENITAL HIP DISLOCATION BY ONE STAGE SURGERY IN 3-5 YEARS OF CHILDREN
Objective: To evaluate the results of a combined single-stage procedure for congenital hip dislocation in a 3 to 5 year group. Study Design: A Descriptive study Place and Duration: The Study was performed in the Orthoped...
AUTOMATED ANALYTICAL TECHNIQUES
Automation is the performance of operation without human intervention. Automation may involve operations likePreparation of samples, Measurements of responses, Calculation of results. There are different automatic method...