Observation of Esterase-Like-Albumin Activity during N’- Nitrosodimethyl amine Induced Hepatic Fibrosis in a Mammalian Model
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2012, Vol 5, Issue 1
Abstract
Aim: Hepatic fibrosis (HF) is characterized by irregular growth and amassing of fibrous scar tissues in the liver causing weakened hepatocytes metabolism and protein level alterations, including albumin. Albumin with M r~68-70 kDa is unglycosylated soluble plasma protein with various biological roles. In this study, we demonstrate ‘esterase-like activity’ of albumin during NDMA-induced HF in rats. Material and Methods: In rats, HF was induced by weekly i.p. injections of NDMA in doses of 10 mg/ kg b.wt. Sera of controls (untreated) and treated rats were processed for biochemical tests, electrophoretic profiling and in-gel esterase activity localization using α, β-naphthyl acetates. H&E staining of liver sections (~ 5 μm) was done to confirm induction of HF. Results: NDMA satisfactorily induces hepatic fibrosis within 21 days which is also evident by significant increase in SALP, SGOT, SGPT and bilirubin levels in rats. ‘Esterase-like activity’ of albumin detected in animal sera remains stable throughout the course of treatment irrespective of other biochemical changes. Conclusion: During pathogenesis of HF, formation of stable esterase-albumin complex may have some important role and hence, prior recommending the use of albumin as diagnostic marker we propose further investigations to elucidate the mechanism of its formation.
Authors and Affiliations
Areeba Ahmad| Biochemical and Clinical Genetics Research Laboratory, Section of Genetics, Department of Zoology, Veena Maheshwari| Department of Pathology, J.N. Medical College, and Hospital, Aligarh Muslim University, Aligarh-202 002 (U.P), India, Arif Ahmad| Department of Biosciences, Jamia Millia Islamia, New Delhi-110 025, Rashid Saleem| Biochemical and Clinical Genetics Research Laboratory, Section of Genetics, Department of Zoology, Riaz Ahmad| Biochemical and Clinical Genetics Research Laboratory, Section of Genetics, Department of Zoology
Keywords
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- EP ID EP8846
- DOI 10.3889/MJMS.1857-5773.2012.0214
- Views 302
- Downloads 15
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