Obtaininig of primary cell culture from freshwater molluscs
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2016, Vol 29, Issue
Abstract
Introduction. Molluscs have the ability to regenerate, but they are not characteristic of malignant tumors. All attempts to get inoculated cell line cultures from the tissue of these animals were not successful. Perhaps the inability of the mollusk body cells segmentation in vitro and low« cancerous potential » causes failure to get finite cell lines. The goal of the work The objective of our work was to obtain primary cell cultures of freshwater bivalves, class bivalves (Bivalvia).Materials and methods. In the study we used three methods enzymatic, mechanical and combination of both methods to obtain primary cell cultures. Observation of cell culture was performed using inverted microscope.Results of research and discussion. Primary culture of freshwater mollusk cultured for 30 days at different temperatures and media. The optimum temperature for cultivation was +20 and+25C°, at a temperature 37C° mollusc cell migration was observed. The largest freshwater molluscs cells output was observed when using the combined method of obtaining primary culture cells. Also, it was observed by using a combination of two culture mediums DMEM and F-12 (1:1)+20% fetal bovine serum.Conclusions and prospects for further research. There were showed results of obtaining of primary freshwater clam culture class bivalves (Bivalvia) by different methods. We used enzymatic, mechanical methods and combination of both methods as well. It was found that the largest freshwater molluscs cells yield was observed using the combined method of obtaining primary culture cells. Also, it was observed when using a combination of two media DMEM and F-12 (1:1)+20% fetal bovine serum. In addition, the optimal temperature +20 and + 25°C was chosen for primary culture culturing.The prospect of further research is to obtain passaged cell culture from freshwater molluscs.The obtained culture can be used in biotechnology, veterinary and humane medicine.
Authors and Affiliations
M. Babkin, Natalia Gavrasieva, G. Kyzmich
Keywords
Related Articles
- EP ID EP274208
- DOI -
- Views 154
- Downloads 0
Biological properties of low pathogenic avian influenza virus H7N3 isolated from wild ducks
Introduction. To date, the influenza virus remains an unpredictable infection for animals, birds and humans. The emergence of new strains and variants with new properties and pathogenicity to new hosts requires constant...
The state veterinary biotechnology in the Republic of Belarus at the modern stage
The development of the national economy of Belarus in the framework of the VI economic order is one of the most preferable and the main ways to solve the problems facing the country at the stage of its entry into a quali...
The retrospective analysis of the leptospirosis rate in Ternopil region
Introduction. At the present time, in Ukraine leptospirosis cases are registered in the territory of all regions of Ukraine and Autonomous republic of Crimea. A basic transmitters and source of leptospirosis are grey rat...
Bacteriological monitoring of infectious diseases of pigs and means of their specific prevention
Introduction. The article presents results of monitoring of infectious diseases of bacteriological etiology in pigs. The basic diseases were determined: colibacteriosis, salmonellosis, pasteurellosis, edema, anaerobic en...
Prevalence of toxoplasmosis among wild boars on the territory of Ukraine
Introduction. The article presents the results of research of wild boar (Sus scrofa) blood serum for the presence of antibodies of toxoplasmosis agent Toxoplasma gondii. The data obtained in the course of an experimental...