Occurrence of Enteric Pathogens on Fresh Produce Grown on Irrigated Soils
Journal Title: Microbiology Research Journal International - Year 2015, Vol 6, Issue 1
Abstract
Aims: To assess the potential health risks of fresh produce grown on irrigated soils treated with manure in Kano State, a large produce region in Nigeria. Methodology: Fresh produce irrigated with wastewater on manure treated soils were assessed for the prevalence, serotype distribution and toxigenicity of Escherichia coli O157, Salmonella spp and Vibrio cholerae in a large produce region in Nigeria. A total of 230 samples obtained from five designated produce locations were examined using selective isolation method with prior enrichment. Fresh produce comprised carrots, cabbage, cucumber, lettuce, tomatoes, spinach, green peas and spring onions. Suspect isolates were identified and characterized by conventional biochemical methods and Microbact 24E (Oxoid, UK) kit. Confirmed isolates were serotyped and E. coli O157 and Vibrio cholerae O1 were assayed for their toxigenic potentials using the Reverse Passive Latex Agglutination kit. The enterotoxigenicity of Salmonella spp was determined by detection of stn gene using polymerase chain reaction (PCR) techniques. Results: Results obtained showed that overall, Salmonella spp and Escherichia coli O157 had the highest prevalence of 17.0% and 10.9%, respectively. Both were most commonly detected from fresh produce. The serotypes of Salmonella detected include Salmonella typhi (51.3%), Salmonella paratyhpi (20.5%) and Salmonella typhimurium (28.2%); strains of Vibrio cholerae O1 detected include Vibrio cholerae O1 of the Ogawa, Inaba and the Hikojima serotypes. Conclusion: The use of untreated irrigation water in vegetable production represents a significant route of transmission of diarrheal pathogen to humans and hence represents a public health risk. We recommend proper and adequate wastewater treatment before use.
Authors and Affiliations
G. O. Abakpa, V. J. Umoh, J. B. Ameh, S. E. Yakubu, J. K. P. Kwaga, A. M. Ibekwe
Keywords
Related Articles
- EP ID EP354693
- DOI 10.9734/BMRJ/2015/13910
- Views 86
- Downloads 0
Change in Antibiotic Susceptibility Pattern of Clinical Bacterial Isolates from Two Hospitals in Dhaka, Bangladesh over a Period of Three Years
Introduction: It is established that improper use of antibiotics leads to rapid development of bacterial antibiotic resistance. We investigated changes in the antibiotic susceptibility pattern of pathogenic bacteria in a...
Effect of Probiotics (Lactobacillus acidophilus and Bifidobacterium bifidum) as Immune Stimulant on Hybrid Catfish Heteroclarias
Study on the effect of probiotics (Lactobacillus acidophilus and Bifidobacterium bifidum) as feed supplement and immune stimulant on hybrid Heteroclarias was carried out with 240 hybrid Heteroclarias fingerlings with mea...
Heavy Metal Tolerance Profile among Bacteria from Auto-mechanic Workshop and Pristine Soil
The study was aimed at investigating the heavy metal tolerance profile among bacteria from auto-mechanic workshop and pristine soil samples. Auto-mechanic workshop and pristine soil environments were randomly sampled wit...
Comparison of two DNA Extraction Protocols Representing Bacterial Community from Bulk Soil by PCR-DGGE
Aims: The objective of this work was to investigate the efficiency of DNA extraction from bulk soil using the Britânia® mini mixer combined with phenol extraction to study bacterial communities, from three sampling sites...
Evaluation of Indigenous Biosurfactant-producing Bacteria for De-emulsification of Crude Oil Emulsions
Conventional method of removing water from crude oil using chemicals is unfavourable from both the economic and environmental perspectives; so, this study aims at formulating economical and environmentally-friendly biosu...