Occurrence of Metallo-Β-Lactmases Producing Pseudomonas aeruginosa among Clinical Isolates
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2018, Vol 6, Issue 2
Abstract
Abstract: Pseudomonas aeruginosa is an increasingly prevalent opportunistic human pathogen and the most common gram negative bacterium found in nosocomial infection. Production of metallo-β- lactamase enzyme in Pseudomonas aeruginosa usually results in high level resistance to most β-lactamases and a rapid spread of MBL producing major gram negative pathogens is a matter of particular concern worldwide. To identify the metallo –β-lactmase production by double disk synergy test, combined disk diffusion method and modified hodge test as described further. Out of 514 samples, on the basis of gram staining , 108(21.01%) gram positive cocci, 296(57.58%) gram negative bacilli, 89(17.31%) no organism seen and 21(4.08%) were yeast.On the basis of culture of 89 samples , 11 (12.35%) were gram positive cocci, 23 (25.84%) were gram negative bacilli, 55(61.81%) were showed no growth. In 319 samples of gram negative bacilli, 100 were isolates of Pseudomonas aeruginosa and in which 18 were found to be producing metallo β-lactmases. This study suggests metallo-beta-lactmase production in pseudomonas aeruginosa is emerging threat in hospital isolates because it is crucial for the optimal treatment of patients.
Authors and Affiliations
Divya Chaubey, Umar Farooq, Sudhir Singh
Keywords
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