OPTIMASI PRODUKSI ANTIBIOTIKA ISOLAT TERPILIH FUNGI ENDOFIT IKD FF-UMI 02 DARI KULIT BUAH DELIMA (Punica granatum L.) DENGAN VARIASI SUMBER KARBON
Journal Title: As-Syifaa Jurnal Farmasi - Year 2017, Vol 9, Issue 1
Abstract
Research of endophytic fungi of pomegranate fruit peel (Punica granatum L.), as Anti-free radicals and antibiotic has been done with DIKTI-PKM funding at 2014. It is considered necessary to further research about optimization of antibiotic production from pomegranate fruit peel with variation of carbon source, first step is selection of isolates that have largest inhibition zone and has the most inhibit microbial test, that is endophytic fungi IKD FF-UMI 02 that has activity on Salmonella thypii, Staphylococcus epidermidis, Staphylococcus aureus and Shigella dysenteriae. Determination of growth curve from isolate by inoculating into Potato Dextrose Broth and incubated for 7x24 hours. Every 12 hours the mycelium is weighed to determine its stationary phase. Determination of optimum conditions of fermentation with carbon source such as glucose, galactose, and fructose. Endophytic fungi IKD FF-UMI 02 inoculated and fermented into production medium which is every 12 hours fermentate was taken. The activity was tested using agar diffusion method in Nutrient Agar (NA) against Salmonella thypii, Staphylococcus epidermidis, Staphylococcus aureus and Shigella dysenteriae. Resulted that growth curve of selected endophytic fungi IKD FF-UMI 02 from pomegranate fruit peel show that stationer phase starting on 192 hour and galactose carbon source is show optimum time of antibiotic production on 120 hours with inhibition zone 10,24 mm against Shigella dysenteriae
Authors and Affiliations
Rusli Rusli, Nurul Vicky Syfriani, Sumarni Hatta, Muh. Wais
Keywords
Related Articles
- EP ID EP540233
- DOI -
- Views 84
- Downloads 0
KOMBINASI EKSTRAK ETANOL DAUN KEMANGI (Ocimum bacilicum L.) DAN DAUN SALAM (Syzygium polyanthum (Wight) Walp) SEBAGAI ANTIINFLAMASI PADA TIKUS (Rattus norvegicus) JANTAN YANG DIINDUKSI KARAGEN
Inflammation is a normal protective response to tissue injury caused by physical trauma that could damage characterized by symptoms such as pain (dolor), heat (kalor), redness (rubor), swelling (tumor) and functional cha...
AKTIVITAS HIPOLIPIDEMIK EKSTRAK ETANOL HERBA KROKOT (Portulaca oleracea L.) PADA TIKUS OBESITAS DENGAN PARAMETER TRIGLISERIDA
Hypolipidemics a drug used to lower plasma lipid levels in the blood. This study aimed to examine the hypolipidemic activity of ethanol extract of purslane herb (Portulaca oleracea L.) against the triglyceride level redu...
PENGEMBANGAN GEL EKSTRAK ETANOL CABAI RAWIT (Capsicum frutescence L)
Chili Pepper (Capsicum frutescence L) is a plant that can be used for the treatment of rheumatoid arthritis (RA). Chemical constituents contained in chili pepper is capsaicin, it was stimulate the nerves that can relieve...
PERBANDINGAN MUTU BERDASARKAN PROFIL DISOLUSI TABLET GLIBENKLAMID PATEN DAN GENERIK YANG BEREDAR DI MAKASSAR
A research of dissolution testing of patent and generic glibenclamide tablet have been done. The purpose of this research is to determine quality of patent and generic glibenclamide tablet that circulated in Makassar. Gl...
AKTIVITAS ANTIBAKTERI EKSTRAK ISOLAT FUNGI ENDOFIT DARI AKAR MANGROVE (Rhizophora apiculata Blume) SECARA KLT BIOAUTOGRAFI
Endophytic fungi have the ability to produce bioactive compounds one of which is antibacterial. A search of bioactive compounds in this research are the first was isolated endophytic fungi on the roots of mangrove then b...