OPTIMIZATION OF ELECTROPORATION MEDIATED TRANSFORMATION OF LACTOBACILLUS PLANTARUM FOR INDUSTRIAL EXPLOITATION
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 8
Abstract
Objective: The objective of our study was to evaluate different established electrotransformation protocols for their suitability on Lactobacillus plantarum.Methods: We tested strategies of electrical parameters in addition to already published electroporation methods proven to be successful in other Lactobacillus strains.Results: Our strain differed highly in transformation efficiencies and optimal growth conditions. Among the various methods performed, we observed high-efficiency protocol yielded 8X106 transformants, and the optimum electroporation parameter was found to be at 1500V.Conclusion: Independent of electroporation method Lactobacillus plantarum strain remained reluctant to high-efficiency transformation protocol.
Authors and Affiliations
Maheswara Reddy Mallu, Siva Reddy Golamari, Sandeep Vemula, Srinivasa Reddy Ronda
Keywords
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