Oral Microbial Ecology of Selenomonas noxia and Scardovia wiggsiae
Journal Title: Microbiology Research Journal International - Year 2017, Vol 21, Issue 3
Abstract
Objectives: Selenomonas species such as S. noxia are associated with poor oral health and oral prevalence of this organism may be useful as a biomarker to determine patient oral health. Current studies are now revealing novel insights into the epidemiology of the newly discovered oral cariogenic organism Scardovia wiggsiae (SW), although few studies have explored the oral microbial ecology with respect to this oral pathogen. Based upon the lack of information regarding the oral microbial ecology, the primary objective of this study was to screen an existing saliva repository to more accurately assess the microbial flora present (or absent) including SN and SW. Experimental Methods: Previously collected saliva samples were evaluated for the DNA isolation and qPCR screening protocol. A total of 42 samples were identified and processed from both pediatric (n=28/42 or 67%) and adult (n=14/42 or 33%) patients. Results: The results of this screening demonstrate that of the SW-positive samples (n=27/42 or 64%) none harbored the oral microbe Selenomonas noxia (SN). Conversely, SN was identified only within the subset of SW-negative samples (n=15/42 or 35%). In addition, although Aggregatibacter actinomycetemcomitans (AA) was only present in a small subset of samples – this organism was only found among SW-positive samples. Other organisms, including T. forsythia (TF), and F.nucleatum (FN) were present in both SW-positive and SW-negative samples although their prevalence differed greatly. Conclusions: This study may be the first to present oral microbial data which suggest SW may participate in direct or indirect bacterial interactions that influence the potential for other organisms to flourish within the oral microbiome. These data suggest that SN and SW may occupy distinct, non-overlapping niches, which may differ significantly from the interactions observed with AA, FN, and TF. Further research will be needed to more fully elucidate these interactions and to explore the potential ramifications for oral microbial ecology and the implications for predictive saliva screening.
Authors and Affiliations
Steven McDaniel, Jaydene McDaniel, Amy Tam, Karl Kingsley, Katherine M. Howard
Keywords
Related Articles
- EP ID EP315892
- DOI 10.9734/MRJI/2017/36110
- Views 129
- Downloads 0
Influence of UV Mutagenesis on β-Mannanase Production Potential of Aspergillus glaucaus and Rhizopus japonicus
Aim: The present study was conducted to improve the β-mannanase production from Aspergillus glaucaus and Rhizopus japonicus using UV mutation. Study Design: The first experiment, spore suspensions of A. glaucaus and R. j...
Diversity of Plasmid Profile in Multi-drug Resistant Non- E. coli Intestinal Flora Lacking Association with Resistance Phenomenon
Aims: To study the plasmid diversity of intestinal non- Escherichia coli community, describe their potential as a reservoir of antibiotic resistance phenomenon and to find any association of resistance phenomenon with th...
Multiple-Antibiotic Resistance in Salmonella enterica Serovars Isolated in Iran Harboring Class 1 Integrons
Aims: This research was carried out to detect the content and distribution of class 1 integrons in multidrug resistant Salmonella isolates. Materials and Methods: Eighty four clinical isolates of Salmonella serovars were...
Characterization of the Genetic Supports for Betalactam Resistance in Escherichia coli Strains of Porcine Origin Producing Extended-spectrum Beta-Lactamase (ESBL)
Aims: The study was to evaluate the genetic potential of beta-lactam resistance in Escherichia coli strains producing extended-spectrum beta-lactamase (ESBL) isolated from the faecal flora of piglets. Place and Duration...
Biochemical and Molecular Characterization of Bacterial Wilt Disease of Banana and Evaluation of their Antibiotic Sensitivity
Aims: Isolation and characterization of bacteria from the bacterial wilt disease of banana and evaluation of their sensitivity. Study Design: This study was an experimental laboratory design. Place and Duration of Study...