Overexpression Analysis of emv2 gene coding for Late Embryogenesis<br /> Abundant Protein from Vigna radiata (Wilczek)
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2008, Vol 4, Issue 3
Abstract
Late embryogenesis abundant (LEA) proteins are speculated to protect against water stress deficit in plants. An over expression system for mungbean late embryogenesis abundant protein, emv2 was constructed in a pET29a vector, designated pET-emv2 which is responsible for higher expression under the transcriptional/translational control of T7/lac promoter incorporated in the Escherichia coli BL21 (DE3).Induction protocol was optimized for pET recombinants harboring the target gene. Overexpressed EMV2 protein was purified to homogeneity and the protein profile monitored by SDS-PAGE.
Authors and Affiliations
S Rajesh, M Raveendran, P Sivakumar, A Manickam
Keywords
Related Articles
- EP ID EP113017
- DOI -
- Views 98
- Downloads 0
EFFECTS OF SALICYLIC ACID ON SEEDLING GROWTH AND NITROGEN METABOLISM IN CUCUMBER (CUCUMIS SATIVUS L.)
Salicylic acid is involved in the regulation of metabolic activity and defense mechanism in plants under various stress conditions. Present study was conducted to determine the effects of salicylic acid (10 to 500 μM) on...
Impacts of Different Water Pollution Sources on Antioxidant Defense Ability in Three Aquatic Macrophytes in Assiut Province, Egypt
The present study was undertaken to evaluate the impacts of surface water pollution with wastes coming from sewage effluents (Site 2), agricultural runoff (Site 4) and oils and detergents factory (Site 3) on the stabilit...
Attenuation of Chromium toxicity in mine waste water using water hyacinth
The mine waste water at South Kaliapani chromite mining area of Orissa (India) showed high levels of toxic hexavalent chromium (Cr[sup]+6[/sup]). Cr[sup]+6[/sup] contaminated mine waste water poses potential threats fo...
Influence of NaCl on Biochemical Parameters of Two Cultivars of Stevia rebaudiana Regenerated in vitro
Soil salinity occupies a prominent place among the soil problems that threaten the sustainability of agriculture over a vast area in the world. It affects plant morpho-physiology and ultimately leads to reduction in prod...
Физиологические отличия симбиотических мутантов гороха с разной степенью нодуляции при инокуляции Rhizobium leguminosarum
Изучали морфологические и физиологические особенности развития растений гороха с разной способностью к нодуляции при инфицировании [i]Rhizobium leguminosarum[/i]. Микроскопические исследования выявили отличия при ф...