PCR detection of Campylobacter fetus subspecies venerealis in smegma samples collected from dairy cattle in Fars, Iran
Journal Title: Veterinary Research Forum - Year 2013, Vol 4, Issue 4
Abstract
Bovine venereal campylobacteriosis, caused by Campylobacter fetus subsp. venerealis (Cfv), is regarded as one of the major threats to the cattle industry around the world. Abortion and infertility are two important reproductive problems in cows infected with C. fetus subsp. venerealis. Reports on the presence of Cfv are scarce in the cattle, in Iran. Therefore, the present study was designed to examine the presence of Cfv in the reproductive tract of dairy cattle either slaughtered in Shiraz abattoir or dairy herds with a history of infertility and abortion, and further to identify and differentiate this micro-organism in dairy cattle in Fars, south of Iran. A total of 95 smegma samples from the preputial cavity and the fornix of the cervical opening were collected using scraping method from bulls (n = 34) and cows (n = 61) in addition to eight samples of commercially bull frozen semen. Smegma samples were then cultured for isolation of Cfv and then the extracted DNA was examined for the presence of Cfv using an optimized multiplex PCR assay. None of the frozen semen samples examined were positive for Cfv. However, out of 95 smegma samples, thirteen animals (12.6%) were found positive for Cfv consisting of 3 males and 10 females. In conclusion, the results of the current study clearly confirmed the presence of Cfv using PCR in the slaughtered cattle and dairy farms with a history of poor fertility and abortion in Fars, Iran.
Authors and Affiliations
Saeid Hosseinzadeh1, Mojtaba Kafi, Mostafa Pour-Teimouri
Keywords
Related Articles
- EP ID EP126010
- DOI -
- Views 95
- Downloads 0
Helminthes and Coccidia Infection of Wild Sheep (Ovis Ammon Orintalis) in Kabodan Island of National Park of Urmia Lake, Iran
Forty-one wild sheep (Ovis ammon orintalis) from Kabodan Island of National Park of UrmiaLake (North-West of Iran), were examined during a period of six months from October 2002 toMarch 2003, for helminthes and coccidian...
Thiopental Sodium Anesthesia Following Premedication of Rabbits with Vitamin C
Rabbits are widely used as laboratory animals for experimental surgery. Anesthesia of rabbits may present complications unless the method is easy to apply and safe to use. In present study, effects of different dosages o...
Effects of diluting medium and holding time on sperm motility analysis by CASA in ram
The aim of this study was to evaluate the effects of dilution rate and holding time on various motility parameters using computer-assisted sperm analysis (CASA). The semen samples were collected from three Ghezel rams. S...
Biogenic amines determination in some traditional cheeses in West Azerbaijan province of Iran
Biogenic amines (BA) are nitrogenous compounds that possess biological activity. The source of production is the microbial decarboxylation of amino acids. This compounds are found in various types of cheese. The ai...