Pecularities of Cytokinin Accumulation and Distribution in Triticum aestivum L. Seedlings Under Temperature Stresses

Abstract

The objective of this research was to investigate the effect of short-term heat (2 h., +40[sup]0[/sup]C) and cold (2 h., +2[sup]0[/sup]C) stresses on accumulation and distribution of cytokinin (CK) in 7 and 14-day-old seedlings of the frost resistant Triticum aestivum L. cultivar Volodarka. A high performance liquid chromatography method has been used to provide a qualitative and quantitative analysis of hormones in leaves and roots. It was shown that in the early stages of growth (7 days) after exposure to high temperature the total content of cytokinin in roots increased 1,6 and in leaves in 2,55 times. The most pronounced changes were registered for trans-zeatin (t-Z) and izopenteniladenozin (iPa) in roots and for zeatinriboside (ZR), izopenteniladenin (iP) and iPa in leaves. After cold stress the total content of hormone in roots increased but decreased in leaves. ZR and iPa were not identified in the leaves. The total level of CK in 14-day-old seedlings as compared to 7-day-old ones under control conditions decreased from 685,3 to 158,3 in roots and from 396,8 to 368,4 ng / g fresh weight in leaves. After cold stress the total content of CK in the roots decreased to 99,9 and in the leaves – to 195,8 ng/g of wet weight. Trans-zeatin was actively accumulated in leaves, ZR - in the roots. After heat stress the total content of CK in roots increased to 316,3, whereas in leaves decreased to 184,6 ng / g fresh weight. Roots actively accumulated cis-zeatin (c-Z) and iPA, leaves – iP and iPa. Changes in the accumulation and distribution of CK depended both on the organs and the age of seedlings, and the type of stress and correlated with frost resistance of the cultivar Volodarka.

Authors and Affiliations

I. Kosakivska, L. Voytenko, R. Likhnyovskiy

Keywords

Related Articles

Screening of Salt-stress, Antioxidant Enzyme, and Antimicrobial Activity of Leave extracts of Mangroves Avicennia marina L. from Hodaidah, Yemen 

In the present study the salinity stress, antioxidant enzyme and antimicrobial activities of leaf extract of Avicennia marinawere investigated. As visualized from SDS-PAGE, no differences was found in number of protein b...

Interactive effects of salinity and low potassium on growth, physiology response of Houttuynia cordata Thunb. W01-100 

Houttuynia cordata Thunb. is a plant enrichment in potassium in plant was reported. Salinity and low potassium availability are important environmental factors restricting plant growth and productivity throughout the wo...

Glutathione is not involved in light-, Dark-, Ca- and H2O2- induced stomatal movement in Arabidopsis

Glutathione (GSH), is a thiol-containing tripeptide, maintains redox homeostasis in plants under normal and stressful conditions. In this study, we investigated whether GSH involved in light-, dark-, Ca- and H[sub]2[/sub...

Protective Effect of Humic acid and Chitosan on Radish (Raphanus sativus, L. var. sativus) Plants Subjected to Cadmium Stress

Background Humic acid or chitosan has been shown to increase plant growth, yield and improving physiological processes in plant, but its roles on alleviating the harmful effect of cadmium on plant growth and some phys...

Comparison the effects of nitric oxide and spermidin pretreatment on alleviation of salt stress in chamomile plant (Matricaria recutita L.)

Salt stress is an important environmental stress that produces reactive oxygen species in plants and causes oxidative injuries. In this investigation, salt stress reduced the shoot and root length, while increased the co...

Download PDF file
  • EP ID EP143843
  • DOI -
  • Views 139
  • Downloads 0

How To Cite

I. Kosakivska, L. Voytenko, R. Likhnyovskiy (2016). Pecularities of Cytokinin Accumulation and Distribution in Triticum aestivum L. Seedlings Under Temperature Stresses. Журнал стресс-физиологии и биохимии, 12(2), 32-38. https://www.europub.co.uk/articles/-A-143843