PHARMACOGNOSTIC AND PHYTOCHEMICAL STUDIES OF CASSIA ABSUS SEED EXTRACTS

Abstract

Objective: To carry out the pharmacognostic and phytochemical analysis of different extracts of seeds of Cassia absus (CA).Methods: The CA plant material (seeds) was studied for its organoleptic, microscopic and physicochemical parameters. The seeds of CA were crushed, and their extraction was carried out in succession with 4 solvents of increasing polarities, viz hexane, ethyl acetate, alcohol, and hydro alcohol. Preliminary phytochemical screening as per standard methods was carried out on all the extracts. Thin layer chromatography of the extracts revealed their qualitative nature. Quantitative estimation of their phenol, flavonoid and sterol contents was carried out. Their antioxidant capacities were evaluated with the help of DPPH and reducing power assays. Their anti-glycation and anti-inflammatory activities were also measured.Results: Qualitative phytochemical analysis showed the presence of alkaloids, flavonoids and steroids in the extracts. The hydroalcoholic extract showed higher phenolic content and DPPH activity among other extracts. The flavonoid, sterol and alkaloid content, and Ferric reducing power were found to be higher in ethyl acetate extract. The hexane extract displayed a higher anti-glycation activity, followed by the ethyl acetate extract.Conclusion: A complete phytochemical screening, along with the evaluation of macroscopic and microscopic characters was carried out for 4 extracts of the seeds of Cassia absus. Quantitative estimation of flavonoid, phenol, sterol and alkaloid content was carried out. The antioxidant, anti-inflammatory, and anti-glycation capacities of the extracts were tested. Alkaloids were successfully extracted from the defatted seeds. 

Authors and Affiliations

Pandita Nancy, Vaidya Ashlesha

Keywords

Related Articles

IN VITRO AND IN SILICO ANTIOXIDANT ACTIVITY OF PURIFIED FRACTIONS FROM PURPLE SWEET POTATO ETHANOLIC EXTRACT

Objective: Purple sweet potato (Ipomoea batatas L.) contains antioxidant compounds like anthocyanins (cyanidin and peonidin). Therefore, the current study was conducted to obtain anthocyanins fractions from purple sweet...

CONDUCTOMETRIC DETERMINATION OF THE ANTIHISTAMINIC DIPHENHYDRAMINE HYDROCHLORIDE USING SILVER NITRATE AS A TITRANT

Objectives: The present study developed and validated a conductometric method for determination of Diphenhydramine HCl (DPH) in its pure form and in a syrup formulation using silver nitrate (AgNO3 ).Methods: Conductometr...

FORMULATION AND EVALUATION OF FLOATING IN SITU GEL OF RANITIDINE USING NATURAL POLYMERS

Objective: To formulate and evaluate floating in situ gel of Ranitidine using natural polymers like sodium alginate and pectin and calcium carbonate as cross-linking agent.Methods: The in situ gel was prepared by mixing...

ANTI-ANEMIC ACTIVITY OF SPROUTS OF VIGNA RADIATA L. IN MALE ALBINO RATS

Objective: To evaluate the anti-anemic activity of sprouts of Vigna radiata L. against phenyl hydrazine induced anemic rats.Methods: Rats were divided into 4 groups of 6 each. Group 1 was given normal saline and served a...

HEPATO-RENAL EFFECTS OF CEFOTAXIME IN ALBINO RATS

Objective: The present study was carried out to evaluate the adverse effects of cefotaxime at two different doses on some biochemical parameters in albino rats. Histopathological changes in liver and kidney induced by ce...

Download PDF file
  • EP ID EP577964
  • DOI -
  • Views 72
  • Downloads 0

How To Cite

Pandita Nancy, Vaidya Ashlesha (2016). PHARMACOGNOSTIC AND PHYTOCHEMICAL STUDIES OF CASSIA ABSUS SEED EXTRACTS. International Journal of Pharmacy and Pharmaceutical Sciences, 8(1), 325-332. https://www.europub.co.uk/articles/-A-577964