PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF FUGA CATTLE BREED BASED ON THE ANALYSIS OF MICROSATELLITES MARKERS
Journal Title: Int J of Pharm Res & Analy - Year 2015, Vol 5, Issue 1
Abstract
A survey was conducted through questionnaire guided interviews with cattle owners (household survey) in selected districts in Fuga cattle habitats. Five villages were selected according to the clustering of villages within the district and accessibility. A set of detailed structured questionnaires were prepared and used to collect information from a total of 150 cattle owners in fuga cattle area. The questionnaires were pre-tested to check clarity and appropriateness of the questions. Some of the information collected during interviews was supported by observation. Milk yield per lactations , milk yield per day ,lactation length , age at first calving ,Calving interval, number of services per conception ,and weight of birth of Fuga cattle breed were presented (1100.96±86.18 kg, 5.26±0.29 kg, 208.60 ± 5.37 days, 40.49, 15.90± 0.44 months, 2.27±0.7 and 22.06±0.44kg) respectively. The population structure and genetic diversity were analyzed using nine microsatellite markers, These markers showed a high level of polymorphism; 64 alleles were identified. The mean number of alleles per locus was 7.11. The mean expected heterozygosity was (0.725) lower than the mean of observed heterozygosity which was (0.778), Polymorphic information content(PIC),Showed substantial variation in PIC among the markers. The mean PIC was 0.664. FST value in all loci was within the average of 0.149.
Authors and Affiliations
Ibrahim H. Ahmed , Abdel Aziz A. Makkawi , Salah-Eldein. Sid-Ahmed , Ahmed S. Abdoon, , Mohammed Saber Hassanane , Sally S Alam
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