Phenotypic detection of metallo-β-lactamase among the clinical isolates of imipenem resistant Pseudomonas and Acinetobacter in tertiary care hospitals
Journal Title: International Journal of Medical Science and Innovative Research (IJMSIR) - Year 2019, Vol 4, Issue 8
Abstract
Background: The rapid spread of Metallo-β-lactamases (MBLs) producing Gram negative bacilli especially Pseudomonas aeruginosa and Acinetobacter species represents a matter of great concern worldwide. The present study was undertaken to analyze the occurrence of MBLs production in Imipenem resistant Pseudomonas and Acinetobacter ssp. isolates. Method: Non fermentative Gram Negative bacilli (NFGNB) were isolated from a variety of clinical specimens, plated on blood agar and MacConkey agar and the Acinetobacter and Pseudomonas species were identified by standard microbiological techniques. The Imipenam resistant strains were subjected to MBL detection by double disk synergy test, (DDST), combined disk test (DPT) and minimum inhibitory concentration (MIC) reduction test. Result: A total of 250 Acinetobacter spp and 180 Pseudomonas spp isolates were obtained from Tertiary Care Hospitals during the period of two years. 44 (17.6%) Acinetobacter strains and 24 (13.3%) Pseudomonas strains were resistant to Imipenem. Amongst 44 screen test positive isolates of Acinetobacter spp., MBL production was detected in 34 (77.27%) isolates each by DDST and MIC reduction test, in 32 (72.72%) isolates by DPT while amongst 24 positive Pseudomonas spp. stains, MBL production was detected in 20 (83.33%) isolates each by DDST and MIC reduction test, in 19 (79.16%) isolates by DPT. Conclusion: MIC reduction and DDSM were better methods for the detection of MBL as compared to DPT. All the microbiology laboratories must routinely screen for imipenem resistance due to MBL, which will help to reduce morbidity and mortality in the patients. Selected use of imipenem and infection control programs for nosocomial infection should also be practiced.
Authors and Affiliations
Dr. Vinay Maniklal Meshram
Keywords
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