Physicochemical Determination Of Ethyl Acetate Extract And N-Hexane Extract Of Azadiractha Indica A. Juss Leaves
Journal Title: International Journal of Health & Medical Research - Year 2024, Vol 3, Issue 01
Abstract
Background: Neem (Azadiractha indica A. Juss) as a tropical and subtropical plant has been widely used in traditional medicine since prehistoric times. Several biological activities, such as antibacterial, anti-inflammatory and antioxidant properties, have been demonstrated in Neem extract. Aim: The study aims to research was carried out on the physicochemical properties of neem leaves (Azadirachta indica A. Juss) using ethyl acetate and n-hexane as solvents. Methode: In this study, Azadiractha indica A. Juss leaves was extracted using a maceration method with two fluids namely ethyl acetate a n-hexane. The extract based on six parameters from determining physicochemical properties, namely % yield, organoleptic, determination of dissolved compound levels, phytochemical screening, determination of drying Shrinkage and determination of specific weight. Result : The results of this study showed, the yield obtained for each solvent is 6.218% ethyl acetate extract and 0.968 % n-hexane extract. Organoleptic examination (form, colour, smell and flavor) for ethyl acetate extract was thick, blackish green, distinctive smell and bitter, and than n-hexan extract was thick, brownish yellow, odorless and bitter. In the ethyl acetate extract, the levels of compounds dissolved in water and ethanol solvents were 16.84 % ± 0.061 and 68.29 % ± 0.042 respectively. The levels of compounds in the n-hexane extract dissolved in water and ethanol were 4.26% ± 0.064 and 23.18% ± 0.049, respectively. The results of the phytochemical screening for ethyl acetate extract of neem leaves are alkaloids and flavonoids. Meanwhile, n-Hexane extract from neem leaves contains alkaloids and terpenoids. The drying loss values obtained from ethyl acetate extract and n-Hexan extract of neem leaves were 4.748% ± 0.257 and 5.219% ± 0.089. Specific gravity obtained from The dilution of ethyl acetate extract and n-hexane extract of neem leaves was 1.0389 g/mL ± 0.039 and 1.0289 g/mL ± 0.038. Conclusion: This study showed that variations in solvents in extraction can affect the physicochemical properties. The extract with ethyl acetate solvent showed better physicochemical properties where the extract obtained in % yield, soluble compound content, phytochemical screening, determination of drying Shrinkage and determination of specific weight was better compared to the extract with n-hexane.
Authors and Affiliations
Yuri Pratiwi Utami , Nurzadrina Wahyuddin , Ainun Jariah , Rahma Mustari , Imelda Desi
Keywords
Related Articles
- EP ID EP731518
- DOI 10.58806/ijhmr.2024.v3i1n01
- Views 44
- Downloads 0
The Challenge of Clinical and Surgical Management of An Acute Myocardial Infarction Simultaneously with An Acute Compressive Neurological Syndrome Due to Pituitary Adenoma: A Case Report
The term acute coronary syndrome represents a group of signs and symptoms in a patient who exhibits clinical and/or laboratory evidence of the occurrence of acute myocardial ischemia. This concept encompasses acute myoca...
Mystery Turn of the Central Venous Catheter!
Anaesthesiologist and intensive care physicians are the best skilled people for placement of central venous catheter. Complications related to Central venous catheter placement have decreased by substantial number due to...
Computational Identification of Human TGFΒ1 Gene: SNPS and Prediction of Their Effect on Protein Functions of Preeclampsia Patients
Growth factor (TGF-β1) belongs to the superfamily (TGF-β) as it has different roles and functions and participates in various physiological, biological, and pathological processes. The protein (TGF-β1) works to regulate...
A Rare Case of Lateral Abdominal Wall Hernia: Case Report and Literature Review
Lateral abdominal wall hernias are rare and complex defects that present unique surgical challenges due to their anatomical location and the underlying musculofascial structures involved. These hernias can result from co...
Identification of Local Clusters of Hand Foot and Mouth Disease Based on Local Geary C Statistic
Background: Hand, foot, and mouth disease (HFMD) is a common childhood illness caused by enteroviruses. Increasingly, the disease has a substantial burden throughout Vietnam. To better understand of the epidemiology of...