Phytochemical and antibacterial studies on the aqueous extract of Eucalyptus gomphocephala DC
Journal Title: Journal of Research in Biology - Year 2014, Vol 4, Issue 8
Abstract
This work aims to find out the molecule responsible for the good activity against the strains of Pseudomonas aeruginosa reflected in a pre work done on the phytochemical study of aqueous extract of Eucalyptus gomphocephala. We have initially processed the quantitative colorimetric determination by a UV-Vis spectrophotometer for total polyphenols and flavonoids and a qualitative analysis by high performance liquid chromatography (HPLC) coupled with mass spectroscopy. Quantitative determinations of total polyphenols by the Folin-Ciocalteu reagent and flavonoids by AlCl3 method revealed the richness of this extract in total polyphenols. Qualitative analysis by HPLC / ESI-MS revealed the presence of gallic acid. This molecule was tested by the agar diffusion method and the macrodilution method in liquid medium, which showed greater activity than the aqueous extract. The results obtained in this study suggest that the gallic acid may be used in the treatment of infections caused by Pseudomonas aeruginosa
Authors and Affiliations
Hayate Bouharb, Khalid El Badaoui, Ali Amechrouq, Jalila El Amri
Keywords
Related Articles
- EP ID EP567253
- DOI -
- Views 74
- Downloads 0
Phytochemical and antibacterial studies on the aqueous extract of Eucalyptus gomphocephala DC
This work aims to find out the molecule responsible for the good activity against the strains of Pseudomonas aeruginosa reflected in a pre work done on the phytochemical study of aqueous extract of Eucalyptus gomphocepha...
DNA barcoding, phylogenetic relationships and speciation of Genus: Plectropomus in Andaman coast
DNA barcode technique has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. The present study carried out for the barcode sequences for identifi...
Assessment of the in vitro antibacterial activity of honey on some common human pathogens
The aim of the present study was to assess the in vitro antibacterial activity of bee honey on certain potentially pathogenic bacterial isolates. Different concentrations (10.0, 20.0, 40.0, 60.0, 80.0 and 100.0%) of hone...
Ethnobotanical plants used as curatives for skin diseases in a Cauvery river stretch, Namakkal district, Tamil Nadu, India
The study area covers about 15 km stretch in the perennial river bed, Cauvery which supports semi evergreen to dry deciduous type of forests. Ethnobotanical information on 24 plant species was recorded during the extensi...
Hepatic enzyme markers and proteins in serum and some selected tissues in Clarias gariepinus from swamp around Kokori-Erhoike oil field, Nigeria
This study determines changes in some biochemical parameters in serum and tissues of Clarias gariepinus obtained from fish natural habitat in the oil exploration environs of Kokori-Erhoike in Delta State, Nigeria. Sampli...