Phytochemical and antibacterial studies on the aqueous extract of Eucalyptus gomphocephala DC
Journal Title: Journal of Research in Biology - Year 2014, Vol 4, Issue 8
Abstract
This work aims to find out the molecule responsible for the good activity against the strains of Pseudomonas aeruginosa reflected in a pre work done on the phytochemical study of aqueous extract of Eucalyptus gomphocephala. We have initially processed the quantitative colorimetric determination by a UV-Vis spectrophotometer for total polyphenols and flavonoids and a qualitative analysis by high performance liquid chromatography (HPLC) coupled with mass spectroscopy. Quantitative determinations of total polyphenols by the Folin-Ciocalteu reagent and flavonoids by AlCl3 method revealed the richness of this extract in total polyphenols. Qualitative analysis by HPLC / ESI-MS revealed the presence of gallic acid. This molecule was tested by the agar diffusion method and the macrodilution method in liquid medium, which showed greater activity than the aqueous extract. The results obtained in this study suggest that the gallic acid may be used in the treatment of infections caused by Pseudomonas aeruginosa
Authors and Affiliations
Hayate Bouharb, Khalid El Badaoui, Ali Amechrouq, Jalila El Amri
Antiparasitic activity of Alstonia boonei De Wild. (Apocynaceae) against Toxoplasma gondii along with its cellular and acute toxicity
Toxoplasmosis (Toxoplasma) and malaria (Plasmodium) are two disease caused by parasites of the phylum Apicomplex. The treatment of toxoplasmosis is similar to that of malaria. Traditionally, Alstonia boonei De Willd. (Ap...
Effect of Pectimorf® - A traditional growth regulator on the development and distribution in clones 'CMC-40' and 'Señorita' of Cassava (Manihot esculenta Crantz) stomata
The development of more efficient and sustainable technologies in the production of materials in ‘in vitro’ cassava (Manihot esculenta Crantz), favor the improvement of seed quality and sanitation of the plant material....
Comparison of different parameters of invasive and non invasive sampling methods for microsatellite genotyping: a case study from Red Junglefowl and its application for other Galliformes
In studies dealing with genetic and disease prognosis, biological sampling is a prerequisite. Earlier, blood and muscle samples were taken after live captures. These invasive procedures are of considerable risk when samp...
Community structure of planktonic copepods in Hendijan harbor (NW Persian Gulf)
The qualitative and quantitative dynamics of the copepod community in Hendijan harbor was studied through zooplankton samples collected seasonally in summer 2010 (July- August), autumn 2010 (October), winter 2011 (Decemb...
In Vitro rapid multiplication of Pergularia daemia - A medicinal plant
A protocol for in vitro multiple shoot development and plantlet formation using leaf explant was prescribed for Pergularia daemia (Forssk.) Chiov. medicinally important plant species. Young apical leaf explant was used f...