Phytochemical and antibacterial studies on the aqueous extract of Eucalyptus gomphocephala DC
Journal Title: Journal of Research in Biology - Year 2014, Vol 4, Issue 8
Abstract
This work aims to find out the molecule responsible for the good activity against the strains of Pseudomonas aeruginosa reflected in a pre work done on the phytochemical study of aqueous extract of Eucalyptus gomphocephala. We have initially processed the quantitative colorimetric determination by a UV-Vis spectrophotometer for total polyphenols and flavonoids and a qualitative analysis by high performance liquid chromatography (HPLC) coupled with mass spectroscopy. Quantitative determinations of total polyphenols by the Folin-Ciocalteu reagent and flavonoids by AlCl3 method revealed the richness of this extract in total polyphenols. Qualitative analysis by HPLC / ESI-MS revealed the presence of gallic acid. This molecule was tested by the agar diffusion method and the macrodilution method in liquid medium, which showed greater activity than the aqueous extract. The results obtained in this study suggest that the gallic acid may be used in the treatment of infections caused by Pseudomonas aeruginosa
Authors and Affiliations
Hayate Bouharb, Khalid El Badaoui, Ali Amechrouq, Jalila El Amri
Keywords
Related Articles
- EP ID EP567253
- DOI -
- Views 73
- Downloads 0
An assessment of bioactive compounds and antioxidants in some tropical legumes, seeds, fruits and spices
Objective: The main objective of this research was to assess bioactive compounds, antioxidant potential and mineral concentration of commonly consumed foods as well as underutilized ones for improved health and food secu...
Wild edible plant Diversity of Kotagiri Hills - a Part of Nilgiri Biosphere Reserve, Southern India
The present study documented indigenous knowledge on wild edible plant resources from the tribe Irulas of Kotagiri at Nilgiri Hills. They are partially or fully dependent on the wildresources for their nutritional requir...
In vitro assessment of water current on growth and biometric relationship among molluscs
Water current plays vital role in the development of an aquatic ecosystem. It performs various activities in the aquatic media, which in turns replenish the nutrients and alter biotic conditions of the water bodies. In o...
Genetic analysis of F2 population of tomato for studying quantitative traits in the cross between Bushbeef x Nagina
This study aims to determine the genetic components like Vg(Variance of genotype), Vp ( Variance of phenotype), GCV (Genotypic co-efficient of variation), PCV (Phenotypic coefficient of variation), Hb (Heritability) and...
Examining the effect of physical, chemical and biological harmful factors on miners
Mining is considered as a difficult and harmful job as it is done in a very difficult physical and environmental condition. In addition to that, miners are exposed to the dangers, damages and even death because of the ba...