PHYTOCHEMICAL, GAS CHROMATOGRAPHY WITH MASS SPECTROMETRY ANALYSIS OF ANDROGRAPHIS SERPYLLIFOLIA METHANOL LEAF EXTRACT AND ITS ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 3
Abstract
Objective: The present study is to evaluate the preliminary study of phytochemical screening and biological applications of Andrographis serpyllifolia methanol leaf extracts. Methods: The methanol leaf extracts of A. serpyllifolia was prepared using Soxhlet apparatus and the extract was analyzed using gas chromatography with mass spectrometry (GC-MS). In vitro antioxidant activity was determined by superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase. Further, the antibacterial activity of methanolic leaf extract of A. serpyllifolia was tested against various human pathogens by using agar disc diffusion method. Results: Preliminary phytochemical screening and GC-MS results revealed phenols, aromatic carboxylic acids, and esters in the chloroform extract to be the molecules responsible for the antioxidant and antibacterial activity of A. serpyllifolia methanol extract and fractions showed the presence of various secondary metabolites present. Conclusion: The present study strongly recommended that the methanolic extract of A. serpyllifolia leaves possesses compounds that inhibit the growth of microbes as wells excellent antioxidant activities. The study further suggested the potential therapeutic use of these extract in cancer study.
Authors and Affiliations
MADHANKUMAR R, MURUGESAN S
Keywords
Related Articles
- EP ID EP603884
- DOI -
- Views 126
- Downloads 0
FORMULATION AND EVALUATION OF NOVEL IN SITU GEL OF LAFUTIDINE FOR GASTRO RETENTIVE DRUG DELIVERY
Objective: The aim of the present study was to formulate and evaluate the novel in situ gel of lafutidine for gastroretentive drug deliveryMethods: A gastroretentive in situ gel of lafutidine was formulated by pH-trigger...
PARVIMONAS MICRA AND FUSOBACTERIUM NUCLEATUM SEPTIC ARTHRITIS: A RARE ANAEROBIC DOUBLE TROUBLE
Septic arthritis is a condition initiated by pathogenic inoculation of joints either by direct or hematogenous route, necessitating immediate medical attention. Among aerobic bacteria, Staphylococcus aureus and Streptoco...
EXPERIMENTAL MODELS FOR HEPATOTOXICITY
The liver is among the most complex and important organs in the human body. Hepatotoxicity is a leading cause of morbidity and mortality, and itsprevalence is continuously increasing day by day in the industrialized nati...
PIPERACILLIN ESTIMATION BY ION-ASSOCIATIVE COMPLEX FORMATION
Objective: The objective of the study was to develop a simple, validated, and affordable visible spectrophotometric method for determination of piperacillin (PIP) present in bulk and powder for injection formulation. Me...
PLACKETT-BURMAN DESIGN AS A TOOL FOR SCREENING AND PROCESS OPTIMIZATION OF RIVASTIGMINE-LOADED LIPID NANOCARRIERS
Objective: Plackett–Burman experimental design is used to identify the most important factors early in the experimentation phase when complete knowledge about the system is usually unavailable. The objective of this st...