PHYTOCHEMICAL INVESTIGATION AND FREE RADICAL SCAVENGING ACTIVITIES OF ESSENTIAL OIL, METHANOL EXTRACT AND METHANOL FRACTIONS OF NEPHROLEPIS BISERRATA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 9
Abstract
Objective: In the current study, the essential oil, methanol extract and methanol fractions (n-hexane, chloroform, ethyl acetate and n-butanol) of Nephrolepis biserrata L. were evaluated.Methods: Preliminary phytochemical screening was done. The antioxidative effect was determined using 2,2diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Total phenol and flavonoid contents were calculated using Folin-Ciocalteau and aluminium chloride reagents. The phytochemical analysis of the essential oil, methanol extract and methanol fractions were performed by gas chromatography - mass spectrometry (GCMS).Results: The preliminary screening confirmed the presence of active chemical constituents such as anthraquinones, alkaloids, tannins, steroids, phytosterol, saponin, triterpinoids and flavonoids. Our results also indicated that essential oil, methanol extract and methanol fractions are rich in phenolic and flavonoid contents. The GCMS analysis of our samples showed the presence of various biological important compounds. The dominant compounds are benzeneacetaldehyde, alpha.-cubebene, butyrolactone, phenol, benzyl alcohol, phenol, 2-methoxy-, 4h-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl, 2h-pyran-2-one, 4,6-dimethyl-, catechol, benzofuran, 2,3-dihydro-, phenol, 2,4-bis(1,1-dimethylethyl), hexadecanoic acid, methyl ester, n-hexadecanoic acid, 9,12-octadecadienoic acid, methyl ester, phytol, gamolenic acid and octadecanoic acid.Conclusion: Our results demonstrate that the essential oil, methanol extract and methanol fractions of the N. biserrata represent a good source of potential bioactive compounds that could be used in pharmaceutical industry.Â
Authors and Affiliations
Muhammad Dawood Shah, Yoong Soon Yong, Mohammad Iqbal
Keywords
Related Articles
- EP ID EP579577
- DOI -
- Views 110
- Downloads 0
IMPACT OF GERANIOL ON CYCLOPHOSPHAMIDE PROVOKED CARDIOTOXIC PROPERTIES–AN IN VIVO STUDY
Objective: Toxicity profiles of diverse anti-neoplastic drugs are extensively documented in past decades and the protective role of natural products against such toxic drugs plays a vital role in the evolution of chemopr...
PRELIMINARY EVALUATION OF AN ANTHRAQUINONE CONJUGATED DOTA DERIVATIVE AS SPECT AGENT
Objective: An anthraquinone derivative, DO3A-Act-AQ having DO3A (1, 4, 7, 10-tetraazacyclododecane-1, 4, 7-trisacetic acid) scaffold is radio labeled with 99mTc radioisotope and evaluated as a SPECT imaging agent for tum...
COMPARATIVE STUDY OF THE ANTINOCICEPTIVE ACTION OF AMITRIPTYLINE WITH FLUOXETINE AND EVALUATION OF THEIR PROBABLE MECHANISM OF THIS ACTION IN ALBINO MICE
Objective: The aim of our study was to compare the anti-nociceptive action of amitriptyline with fluoxetine and evaluation of their probable mechanism of anti-nociceptive action by observing their individual interactions...
PHARMACOEPIDEMIOLOGICAL SURVEY ON THE USE OF COSTUS PICTUS (INSULIN PLANT) IN CENTRAL KERALA
Objective: Costus pictus commonly known as spiral ginger or insulin plant is grown in gardens as ornamental plant especially in Kerala and used to control sugar levels. The objective of the present survey was to collect...
ANTIDEPRESSANT ACTIVITY OF AQUEOUS EXTRACTS OF FRUITS OF TERMINALIA CHEBULA AND PHYLLANTHUS EMBLICA IN BEHAVIOURAL MODELS OF DEPRESSION: INVOLVEMENT OF MONOAMINERGIC SYSTEM
Objectives: Terminalia chebula (TCh) and Phyllanthus emblica (PE) posses wide range of central nervous system activity. This study was conducted to investigate the antidepressant activity and mechanism action of aqueous...