Polymorphism A/C in exon 7 of the bovine estrogen receptor α (ERα) gene and its association with functional and milk production traits in Red-and-White cattle
Journal Title: Animal Science Papers and Reports - Year 2011, Vol 29, Issue 4
Abstract
Due to the functions that estrogens play in the regulation of reproduction, development of mammary gland, growth and differentiation of cells, estrogen receptors and their genes are considered candidates for molecular markers of production and functional traits in farm animals. In this study, using the SSCP and DNA sequencing, we found a novel single nucleotide polymorphism (SNP) in the coding region of the estrogen receptor α (ERα) gene – the A/C transversion at position 323,396 (relative to the start of transcription site), in exon 7, that could be also detected with RFLP-CfrI. This mutation causes the amino acid replacement – Asparatic acid/Alanine in the ligand-binding domain (LBD) of the receptor. The ERα A/C (RFLP-CfrI) genotypes were estimated in a cohort of 489 cattle of different breeds, including 355 Red-and-White cows. Association was studied between ERα genotype and dairy production traits (milk yield and composition) and functional traits (reproduction, length of productive life). The results showed that ERα A/C genotype affected significantly only a few traits of interest: protein and fat content in milk, sex of calves born. No associations were detected between ERα genotype and milk yield and reproduction traits of Red-and-White cows.
Authors and Affiliations
Tomasz Szreder, Jolanta Oprządek, Beata Żelazowska, Edward Dymnicki, Lech Zwierzchowski
Keywords
Related Articles
- EP ID EP71327
- DOI -
- Views 144
- Downloads 0
The effects of polymorphisms in DGAT1, GH and GHR genes on reproduction and production traits in Jersey cows
The aim of the study was to examine the impact of four single nucleotide polymorphisms in DGAT1, GH and GHR genes on reproduction, production and udder health in Jersey cattle. The study was conducted on 209 cows from th...
In vitro supplementation of lycopene to bovine spermatozoa: effects on motility, viability and superoxide production
This study was designed to assess the effects of lycopene (LYC) on motility, viability and superoxide production of bovine spermatozoa during short-term (0h, 2h, 6h) and long-term (12h, 24h) in vitro culture periods. Sem...
The effect of mesenchymal stem cells as co-culture in in vitro nuclear maturation of ovine oocytes
This study compared the effects of ovine mesenchymal stem cells (MSCs) and ovine oviductal epithelial cells (OECs) as feeder cells in cell free culture systems (HEPES-modified tissue culture medium, TCM199) supplemented...
Power of different microsatellite panels for paternity analysis in sheep
The aim of this work was to develop and to test PCR-based suitable microsatellite marker panels for paternity testing in sheep. Blood samples were collected from 212 Kivircik sheep (24 rams and their 188 offspring) rais...
The effect of organic vs. conventional rearing system on performance, carcass traits and meat quality of fast and slow growing rabbits
The effect of different housing systems was evaluated on productive performances and carcass and meat quality of a “local grey” population of rabbits (G). To compare data obtained from G, commercial hybrid rabbits (H) we...