Potential Extract of Lerak Fruit (Sapindus Rarak), Pegagan (Centella Asiatica,) Mimba Seed (Azadirachta Indica A.Juss) and Mixing of Third Extract to Motility and Viability of Spermatozoa Guinea Pigs (Cavia Porcellus)
Journal Title: International Journal of Health & Medical Research - Year 2023, Vol 2, Issue 06
Abstract
Background: Spermicides are substances that can kill spermatozoa. Spermicide circulating in the community contains Nonoxynol9 which, if used for a long time, can irritate the vaginal mucosa and penis. Indonesia has a variety of plants that have the potential to act as anti-fertility, including Sapindus rarak, Centella asiatica, and Azadirachta indica. Objective: This study aim to evaluate extract Sapindus rarak, Centella asiatica, and Azadirachta indica. and mixing of third etract to motility and viability of spermatozoa Guinea pigs. Methods: The sample of this research was 15 guinea pig spermatozoa, 3-4 months old male guinea pigs taken from the cauda epididymis and vas deferens. Guinea pigs were divided into 5 group treatments with each group treatment incubated for 1, 10, and 30 minutes and repeated each for 5 times. The treatments in this study is control, 200 μl of spermatozoa suspension + 50 μl of Sapindus rarak fruit pulp extract 0.9%; 200 μl of spermatozoa suspension + 50 μl of gotu kola extract 0.9%; 200 μl of spermatozoa suspension + 50 μl of 0.9% neem seed extract; 200 μl of spermatozoa suspension + 50 μl of the third mixture extract 0.9%. This study used a completely randomized design (CRD). The observations in this study were the motility of guinea pig spermatozoa categories a + b and the viability of guinea pig spermatozoa using a microscope. The data obtained were analyzed statistically using One Way ANOVA. The results of this study indicated that the administration of extract Sapindus rarak, Centella asiatica, and Azadirachta indica and a mixture of the three extracts had a significant effect (P <0.05) on the motility of guinea pig spermatozoa categories a + b and viability of guinea pig spermatozoa. Conclution: This study concludes that the mixture of the three extracts has more potential in reducing the motility of spermatozoa categories a + b and the viability of guinea pig spermatozoa compared to single extracts. The mixture of the three extracts may contribute for reduce quality of sperm.
Authors and Affiliations
Sukarjati , Erni Widyaswati , Intan Ayu Pramusinta , Asti Rahayu
Keywords
Related Articles
- EP ID EP730914
- DOI 10.58806/ijhmr.2023.v2i6n02
- Views 53
- Downloads 0
The Impact of Exclusive Breastfeeding Promotion on Increasing The Interest of Pregnant Women in Exclusive Breastfeeding
Introduction: Promotion of exclusive breastfeeding is one of the important efforts to increase breastfeeding practices. However, the extent to which the promotion of exclusive breastfeeding affects the interest of pregna...
Vitamin D Deficiency And Risk Of COVID-19 Infection
There is an urgent need for new supportive treatment options against infectious viral diseases, given the rapid spread and huge number of individuals affected worldwide by these diseases, so cost-effective, globally avai...
Pharmacological Interrelationship Analysis between Terminalia chebula and HIV: A Pathway Analysis
Background: It's intriguing to learn about the various ways traditional plants can be used to treat medical issues. Many studies are being undertaken to see how traditional herbal fruits can help with a variety of medic...
Laboratory Tests for The Diagnosis of Breast Cancer
Breast cancer (BC) has posed a significant threat to the general health of women globally, with its incidence and mortality rates showing a noticeable increase in China over time. Research indicates that the survival rat...
Exploring In-School Adolescent Smoking Behaviors: A Comparative Study of Rural and Urban Contexts in Anambra, Nigeria
The use of tobacco among adolescents is a big public health issue in low- and middle-income countries of the world including Nigeria. The aim of this study was to compare prevalence and determinants of tobacco use among...