Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats
Journal Title: Journal of Applied Veterinary Sciences - Year 2017, Vol 2, Issue 1
Abstract
To examine the effect of osteopontin inhibition by tranilast on liver fibrosis, four groups of rats were used throughout this study. Group I (Control group): rats received the solvent. Liver fibrosis was induced in Groups II, III, and IV by thioacetamide (TAA; 200mg/kg, ip) twice weekly for 6 weeks. Group II served as (TAA group). Groups III and IV (Treatment groups): rats were given tranilast for 6 weeks after TAA discontinuation. Liver osteopontin (OPN), transforming growth factor-β (TGF-β1), tumour necrosis factor-alpha (TNF-α), alpha-smooth muscle actin (ɑ-SMA)), reduced glutathione (GSH), superoxide dismutase (SOD) and lipid peroxidation (MDA) were measured. Additionally, the expression of α-smooth muscle actin (SMA) and caspase (Cas)-3 were assigned immunohistochemically. Treatment with tranilast prevented the development of hepatic fibrosis and the activation of stellate cells, and down-regulated the expression of genes for osteopontin and osteopontin-target molecules, including TGF-β and TNF-α and α-SMA. Tranilast significantly decreased MDA and increased levels of GSH, and SOD. Our findings suggest that targeting osteopontin with tranilast represents a new model of therapy for liver fibrosis.
Authors and Affiliations
Ramadan A. , Nehal A. Afifi , Nemat Z. Yassin, Rehab F. Abdel-Rahman , Sahar S. Abd El-Rahman, Hany M. Fayed
Keywords
Related Articles
- EP ID EP677177
- DOI 10.21608/JAVS.2017.62129
- Views 341
- Downloads 0
Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria
This study was conducted to determine the occurrence and antimicrobial susceptibility of Staphylococcus piscifermentans nasal colonization among apparently healthy ruminants and their handlers in Maiduguri, Borno State,...
Review On The Common Surgical Affections In Sheep And Goats
Numerous surgical affections are common among sheep and goats in different localities all over the world which may cause economic losses and most of these affections are curable. Neglection or late interventions to reli...
Preliminary Studies On The Virus Causing Outbreak Of Duckling Short Beak And Dwarfism Syndrome (Sbds) In Egypt
Many Mule, Muscovy, and Bikini duck flocks in different duck-producing regions in Egypt have shown signs of a new disease designated "short beak and dwarfism syndrome" (SBDS) since 2015. The ducks with SBDS showed strong...
Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats
To examine the effect of osteopontin inhibition by tranilast on liver fibrosis, four groups of rats were used throughout this study. Group I (Control group): rats received the solvent. Liver fibrosis was induced in Group...
Improvement Of Inactivated Equine Herpes Virus-1 Vaccine Using Carbomer
An inactivated Equine herpes virus-1 vaccine was successfully improved using carbomer as adjuvant inducing high and long immunity in vaccinated mares in comparison with the convention one adjuvanted with Al-hydra gel and...