Prediction of protein structure of novel protein (116 kDa) from human sperm membrane
Journal Title: Asian Pacific Journal of Reproduction - Year 2018, Vol 7, Issue 3
Abstract
Objective: To predict the protein similarity, biological function and structure of 116 kDa protein that was isolated from human sperm membrane of acrosome by using in silico. Methods: Predictions for 116 kDa protein similarity was done through comparative analysis of its isoelectric point (pI) and molecular weight to the UniProtKB/TrEMBL protein database. The three-dimensional structure of the protein was built using SWISS-MODEL. Results: The 116 kDa protein with pI 4.4 was expected to have similarities with CDH23 proteins that had pI 4.38, thus they likely had similar physical and biochemical properties. The predicted protein had an extracellular N-terminal that likely acted as a receptor, and showed cell recognition characteristic, playing a role in fertilization. Conclusions: The 116 kDa protein isolated from acrosome membrane has similarity on its biophysical character to the CDH23.
Authors and Affiliations
Umie Lestari, Widodo Widodo, Sutiman Bambang Sumitro
Keywords
Related Articles
- EP ID EP407604
- DOI 10.4103/2305-0500.233575
- Views 209
- Downloads 0
Effects of stem cells applications on oxidative stress and apoptosis during implantation
Objective: To investigate the effects of bone marrow derived mesenchymal stem cell (BMSC) application into the rat endometrium on oxidative stress, cell proliferation and apoptosis. Methods: The female rats selected in e...
The methanolic extract of Guibourtia tessmannii (Caesalpiniaceae) improves sexual parameters in high fat diet-induced obese sexually sluggish rats
Objective: To evaluate the effects of the methanolic extract of Guibourtia tessmannii (G. tesmannii) on sperm parameters, lipid profile and testosterone level in obese rats. Methods: A total of 193 male Wistar rats were...
Platelet indices in evaluation of patients with recurrent pregnancy loss
Objective: To explore the change of platelet indices namely plateletcrit, platelet distribution width and mean platelet volume among patients with recurrent pregnancy loss (RPL). Methods: The medical records of 45 women...
Impact of replacing egg yolk with lecithin on quality of pre-freeze and post-thaw buffalo spermatozoa
Objective: To estimate the result of egg yolk replacement with alternative cryopreservatives such as plant-derived lecithin from soybean on sperm quality parameters pre and post freezing in buffalo bulls. Methods: The co...
Pregnancy outcome of day 3 versus day 5 embryo transfer: A retrospective analysis
Objective: To compare the pregnancy rate between day 3 and day 5 transfer regardless grades of embryos and number of transferred embryo. Methods: Retrospective cohort, a total of seven hundred and four patients met our i...