Preliminary investigation on the combined effect of S-adenosyl-L-methionine (SAM) and oseltamivir on experimental influenza А virus infection in mice
Journal Title: Journal of BioScience and Biotechnology - Year 2016, Vol 5, Issue 3
Abstract
Influenza is one of the most contagious viral diseases, caused by influenza virus and it affects thousands of people every year. The infection causes changes in the intracellular redox balance, increased production of reactive oxygen species, development of antioxidant deficiency and conditions of oxidative stress. Decreased level of gluthatione during flu is responsible for the severe pathology and complications. The purpose of our studies was to follow the effect of the combination S-adenosyl-L-methionine (SAM) as a precursor of glutathione and the specific neuraminidase inhibitor oseltamivir in influenza infected mice. SAM was given as a single daily dose of 50, 100 and 150 mg/kg, starting from 5 days before infection until day 4th after viral inoculation. Oseltamivir was given in a daily dose of 2.5 mg/kg in two intakes for 5 days, starting from 4th hour before infection. End-point evaluation was 14 day survival rate, average survival time, index of protection, and virus titer in lungs. The results showed that application of SAM alone did not indicate significant antiviral protection. In mice supplemented with oseltamivir only survival rate was 70%, but combination of oseltamivir and SAM in lower doses led to rising of 90% protection. The present findings suggest that combined therapy of SAM as a precursor of glutathione and the specific inhibitor of influenza virus replication oseltamivir could be effective on modulation of host defence mechanism(s) in low therapeutic doses.
Authors and Affiliations
Milka Mileva, Dimo Krastev, Adriana Dimitrova, Angel Galabov
Keywords
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