Preparation and application of multicolor luminescent lanthanide nanoparticles
Journal Title: Science Paper Online - Year 2009, Vol 4, Issue 9
Abstract
Because strongly fluorescent lanthanide compounds are mainly limited to Eu3+ and Tb3+ compounds with red (Eu, ~615 nm) and green (Tb, ~545 nm) emissions, the development and application of fluorescence bioassay technique using lanthanide-based multicolor fluorescent biolabels are rarely investigated. A series of lanthanide complexes-bound silica nanoparticles with red, orange, yellow and green fluorescence colors was prepared by co-binding different ratios of Eu3+/Tb3+ fluorescent complexes with the ligand (4’-phenyl-2,2’:6’,2”-terpyridine-6,6”-diyl) bis(methylenenitrilo) tetrakis (acetic acid) inside the silica nanoparticles. The nanoparticle-labeled streptavidin was prepared and used for time-resolved fluorescence detections of human prostate-specific antigen and an environmental pathogen Giardia lamblia. The results indicate that the new nanoparticles are useful to multicolor biolabels for time-resolved fluorescence bioassays.
Authors and Affiliations
Hongfei Jiang, Zhiqiang Ye, Xiaoyu Liu
Keywords
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