PREPARATION, DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF APIGENIN IN APIGENIN–HYDROGENATED SOY PHOSPHATIDYLCHOLINE (HSPC) COMPLEX

Abstract

Objective: To Develop simple, sensitive UV – visible spectrophotometric method for determination of Apigenin (APG) in Apigenin – Hydrogenated Soy Phosphatidylcholine (HSPC) Complex.Methods: The APG –HSPC Complex (phytosomes) were prepared by dissolving both APG and HSPC in 20 ml mixture of 1, 4 – dioxane: methanol at a ratio of (14:6) by refluxing and complex produced by solvent evaporation method. The spectrophotometric detection of APG was done at the absorption maximum (λ max) of 335 nm and 268 nm using methanol as solvent. The developed method was validated as per International Conference on Harmonization (ICH) guidelines.Results: APG content in APG – HSPC Complex was found to be 82.86±0.90% and 76.89±0.84% at 335 nm and 268 nm. APG exhibited good linearity in concentration range 2 – 12 µg/ml (r2>0.99) at 335 nm and 2 – 14 µg/ml (r2>0.99) at 268 nm. Precision and mean recoveries were found to be in the range of (% RSD 0.0981 & 0.0989) and (% RSD 0.0829 & 0.1116) and 94.67±2.52 % & 86.56±1.90 % at 335 nm and at 268 nm. The limit of detection (LOD) and limit of quantification (LOQ) was found to be (0.0106µg/ml & 0.0322µg/ml) and (0.0259µg/ml & 0.0757µg/ml) respectively.Conclusion: The developed method was found to be minimal, specific, economic, reliable, accurate, precise, and reproducible that used as a quality control tool for analysis of APG. 

Authors and Affiliations

Arun P, Amol T, T Darshan

Keywords

Related Articles

COMPARATIVE STUDY OF KANAMYCIN AND CAPREOMYCIN ON SERUM POTASSIUM LEVEL OF MULTIDRUG RESISTANCE TUBERCULOSIS PATIENTSAT A HOSPITAL IN BANDUNG, INDONESIA

Objective: Kanamycin and capreomycin are the second lines injectable ant tuberculosis used in therapy regimen of multidrug resistance tuberculosis (MDR TB) in Indonesia. Both have effects on serum potassium levels and ca...

AN IMPLICATION OF ACTINOMYCETES ON HUMAN WELL-BEING: A REVIEW

This review conceptualizes about the actinomycetes and its contribution to human health by playing a key role as bioactive secondary metabolites, such as enzymes, antibiotics and pigments, leading to their diverse applic...

ESTIMATED DAILY INTAKE AND EXPOSURE OF SODIUM BENZOATE AND POTASSIUM SORBATE THROUGH FOOD PRODUCTS IN SCHOOL CHILDREN OF TIRUPATI, INDIA

Objective: According to Food Safety and Standards Authority of India (FSSAI) the preservatives, sodium benzoate and potassium sorbate belong to permitted class II preservatives. The aim of this study is to determine the...

UV SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF ACAMPROSATE CALCIUM IN TABLETS

Objective: To develop a rapid UV spectrophotometric method for the quantitative estimation of Acamprosate calcium (333mg) in tablets and validate as per ICH guidelines.Methods: The optimized method uses a diluent 100% Tr...

IN-SILICO DESIGN, SYNTHESIS AND IN VITRO ANTITUBERCULAR ACTIVITY OF NOVEL 1, 2, 4 - TRIAZOLE DERIVATIVES

Objective: To design, synthesize and in vitro anti tubercular evaluation of some new 1,2,4–triazole derivatives.Methods: Novel 1,2,4–triazole derivatives were designed by using various softwares like ACD Lab Chemsket...

Download PDF file
  • EP ID EP578964
  • DOI -
  • Views 101
  • Downloads 0

How To Cite

Arun P, Amol T, T Darshan (2015). PREPARATION, DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF APIGENIN IN APIGENIN–HYDROGENATED SOY PHOSPHATIDYLCHOLINE (HSPC) COMPLEX. International Journal of Pharmacy and Pharmaceutical Sciences, 7(3), 228-231. https://www.europub.co.uk/articles/-A-578964