Preparation of nanohybrid antibiotic from levofloxacin and determination its inhibitory efficacy against Staphylococcus aureus isolated from diabetic foot ulcer
Journal Title: Scientific Journal of Medical Research - Year 2018, Vol 2, Issue 5
Abstract
Objective: This study aimed to Prepare nano hybrid from levofloxacin antibiotic and evaluation of its inhibitory effect against Staphylococcus aureus from diabetic foot ulcer. Methods: A total 43 swab samples were collected from diabetic foot ulcer patients hospitalized in AL-Hussein Teaching Hospital in Sacred Kerbala province, it has been obtained 9 isolates were Staphylococcus aureus. A nanohybrid antibiotic LEV-LDH was prepared using direct ion exchange between antibiotic levofloxacin (LEV) and layered double hydroxide (LDH). The new nano antibiotic was identified. FT-IR spectroscopy revealed the appearance of new groups in the LEV-LDH spectrum, which indicates the formation of a new compound. The X-Ray Diffraction (XRD) spectrum revealed the appearance of new diffraction planes in the nanohybrid antibiotic spectrum in compare with spectrum of layered double hydroxide. The two and three-dimensional image of the above compound in atomic force microscope confirmed the formation of new antibiotic with nano dimensions. The antimicrobial activity of the nanohybrid antibiotic was studied against 9 isolates of S. aureus. Results: The results showed that the highest inhibition was obtained against isolate 3 with average inhibition zone of 34.642 mm. The combination between the antibiotics LEV-LDH and teicoplanin was studied against S. aureus bacteria, the results showed a synergistic inhibition effect against some isolates while others gave antagonist inhibition effect. Conclusion: The efficacy of the nanohybrid levofloxacin antibiotic in inhibition of isolated S. aureus.
Authors and Affiliations
Sara M. AL-Hussaini, Ali Abdul Kadhim Al-Ghanimi, Hesham Ibrahim Kadhim
Keywords
Related Articles
- EP ID EP283375
- DOI -
- Views 155
- Downloads 0
First record of myiasis case by Stomoxyscalcitrans Linnaeus (Diptera:Muscidae) in Karbala Governorate on pigeon chick
Objective: The aim of current study was to investigate taxonomic and description cases of myiasis recorded of survey regions in karbala. Methods: Survey regions karbala for collected flies larvae Myasis and...
The analysis of the protective feature of Nigella sativa in reducing Carbimazole toxicity including liver and kidney parameters on Albino male rats
Objective: Carbimazole is widespread drug utilized for treating hyperthyroidism but ,carbimazole usage was associated with adverse on some organs. Also, carbimazole overdose has been linked to nephritis in rats, while ,...
Helicobacter pylori CagA antigen detection of gastric infected patients and its association to severity of disease in Iraq
Objective: Helicobacter pylori (H.pylori) is associated with various upper gastrointestinal tract disorders. Many studies have measured anti-cytotoxin-associated gene A (CagA) antibody concentration as signs of CagA anti...
STUDY OF USING KOJIC ACID AS ANTICANCER IN DIFFERENT CONCENTRATION TO TREATMENT CANCERED RATS Rattus norvegicus BY CYCLOPHOSPHAMIDE
Objective: This study was conduct to investigate effect of kojic acid to treatment ofcancered rate. Methods: Different concentration 0.5, 1, 2 mg/kg used as treatment through 30 days in some histological and functional c...
Histopathological Study Of Toxicity Aflatoxin Of Treatment By Aqueous Extract Seed Pomgranate (Punica granulatum) In Male New Zealand White Rabbits (Oryctolacus cunnculus)
Objective: The current study focus on toxicity of aflatoxin of treatment by Aqueous extract seed pomgranate (Punica granulatum) in male New Zealand white Rabbits (Oryctolacus cunnculus) until we show the effect of three...