Prevalence and Antimicrobial Resistance of Campylobacter Species Isolated from Backyard Chickens in Grenada, West Indies
Journal Title: Microbiology Research Journal International - Year 2016, Vol 11, Issue 4
Abstract
Aim: This study was carried out to assess the prevalence of Campylobacter spp. in free range chickens in Grenada, West Indies and to determine the antimicrobial susceptibility of isolates. Study Design: Cloacal swabs were collected from 315 free range chickens from randomly selected households from all six parishes of Grenada between June and July 2014. Cloacal swabs were cultured for Campylobacter in the Pathobiology Diagnostic Laboratory, School of Veterinary medicine, St. Georges University Grenada. Isolates were further tested through PCR for speciation. Methodology: Standard culture methods for isolation of Campylobacter spp were used. Isolates were characterized by conventional phenotypic tests and confirmed by PCR using species specific primers. The 16s rRNA gene for Campylobacter spp.; the map A gene for C. jejuni and the ceuE gene for C. coli were selected for PCR. Isolates were tested through E-test for their antimicrobial susceptibility using Ampicillin, Ciprofloxacin, Chloramphenicol, Erythromycin, Gentamicin, Metronidazole and Tetracycline. Results: A total of 158 isolates (50.2%) were obtained by culture. PCR on 93 isolates identified 33 isolates as C. jejuni, 6 as C. coli and 54 as mixed infection with C. jejuni and C. coli. None of the isolates was resistant to chloramphenicol and erythromycin; susceptibility to other antimicrobials varied among isolates. Multidrug resistance was high in C. coli (33.3%), followed by mixed infection isolates (22.2%) and C. jejuni (12.0%). Conclusion: Results of the study show that approximately 50% of backyard chickens in Grenada harbor Campylobacter spp. These backyard chickens pose a great risk for humans as hazard analysis critical control point (HACCP) is not observed during the slaughter and processing of these chickens.
Authors and Affiliations
Ravindra Sharma, Keshaw Tiwari, Vanessa Mathew Belmar, Sunil Kumar, Sagar M. Goyal, Victor A. Amadi, Natalie Watson, Harry Hariharan
Keywords
Related Articles
- EP ID EP350775
- DOI 10.9734/BMRJ/2016/21741
- Views 98
- Downloads 0
Production of Fungal Laccase Using Orange Peelings as Substrate by Submerged Static Fermentation
Aims: Laccases are diphenol oxidases that have numerous applications in biotechnological processes. In this work, the production of fungal laccase using organic and inorganic feed substrates in submerged static fermentat...
Probiotic Properties Profiling of Isolated Lactic Acid Bacteria from the Intestine of Channa punctata
Aims: This study was conducted for isolation, identification and probiotic profiling of Lactobacillus spp. from the intestine of indigenous and non-indigenous Channa punctata species. Study Design: Lactobacillus spp. fro...
Assessing the Impact of Antiretroviral Therapy on CD4, Hemoglobin Level and Weight in HIV Infected Children (0-15 Years) Residing in Nkambe, North West Region, Cameroon
Background: Antiretroviral therapy (ART) has greatly decreased the mortality and morbidity of children living with HIV by reducing the viral load, increase CD4 count thus improving the health of HIV clients. This study s...
Ring Worm Skin Infection in a Rural Community of Bushenyi (Uganda)
Aim: Ring worm skin infections which have continuously been neglected in several communities of sub Saharan Africa, thus leading to the development of herbal remedies as communities seek to find solutions to the menace....
Molecular Epidemiology of Foot-and-Mouth Disease Viruses Circulated in Bangladesh from 2011–2014
Background: Foot-and-mouth disease (FMD) is one of the highly contagious and economically devastating diseases of cloven-hoofed animals in Bangladesh. The present study was undertaken to investigate the molecular epidemi...