Process optimization of supercritical carbon dioxide (SC-CO2) extraction parameters for extraction of deoxynojirimycin (1-DNJ) from mulberry (Morus alba L.) leaves
Journal Title: Journal of Applied and Natural Science - Year 2016, Vol 8, Issue 1
Abstract
In the present study, supercritical fluid extraction (SFE) technology was applied to extract deoxynojirimycin (1-DNJ) from mulberry leaf powder using carbon dioxide (CO2) as major extraction solvent with ethanol as cosolvent, and extraction parameters such as pressure (100, 150 and 200 bar), temperature (40, 50 and 60 °C) and dynamic extraction time (40, 60 and 80 min) were systematically investigated by full factorial design to obtain the optimum extraction efficiency and extraction yield. Under optimized conditions (pressure of 200 bar, temperature of 50 °C and dynamic extraction time of 80 min), DNJ enriched extract was obtained with high extraction efficiency (96.46 %) and extraction yield (13.41 %), enabling this product to use for nutraceutical purpose. The results indicated that SC-CO2 extraction is a promising and alternative process for recovering the bioactive compounds from mulberry leaves.
Authors and Affiliations
V. Ramya, Udaykumar Nidoni, Sharangouda Hiregoudar, C. T. Ramachandra, J. Ashoka
Keywords
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