Prognostic Value of KRAS Mutations in Codons 12 and 13 in South Egyptian Colorectal Cancer Patients
Journal Title: Journal of Cancer and Tumor International - Year 2015, Vol 2, Issue 4
Abstract
Aims: The aim of the study was to assess the frequency of Kirsten rat sarcoma viral oncogene homolog (KRAS) gene mutation, perform clinicopathologic characterization of KRAS mutated colorectal cancer (CRC), evaluation of the prognostic significance of KRAS mutations and their influences on the outcome in patients with CRC after curative surgical resection. Patients and Methods: This study included 79 previously untreated CRC cases presented to South Egypt Cancer Institute from January 2011 to June 2013. Tumor samples were prospectively analyzed for KRAS mutations detection using DNA Clamp technique. Results: Genotyping revealed an overall mutation frequency of 31.6% in KRAS gene. 68% of the mutations were in codon 12 and 23% in codon 13. Patients with KRAS mutated CRC had statistically significant increase in the rate of local recurrence (P=.002) and distant metastases (P=.024) compared to patients with KRAS wild type CRC. There was statistically significant poorer 2-year disease free survival (DFS) (61.6% vs 86.2%, P=.01) and overall survival (OS) (83.1% vs 90.7%, P=0.04) in cases with KRAS mutated vs KRAS wild type CRC. Codon 13 was associated with statistically significant lower 2-year DFS (46.9% vs 81.4%, P=.008) and OS (62.5% vs 91.4%, P=.009) compared to cases without codon 13 KRAS mutation. Conclusion: The prognostic significance of KRAS mutation status regarding survival and treatment outcome in CRC might form the basis for more specific adjuvant therapy to improve survival.
Authors and Affiliations
Alia M. Attia, Hosny Badrawy, Hisham M. Hamza, Tarek M. Elsaba
Keywords
Related Articles
- EP ID EP242996
- DOI 10.9734/JCTI/2015/20417
- Views 95
- Downloads 0
Role of Breast Feeding and Parity in Prevalence of Breast Cancer and Bone Metastasis
Our aim was to evaluate the role of breast feeding and parity (alive and abortion) in prevalence of breast cancer and secondary bone metastasis. For The study purpose 100 diagnosed females of breast cancer were selected....
Efficacy and Safety of Sorafenib in Patients with Advanced Hepatocellular Carcinoma
Aims: Advanced Hepatocellular carcinoma (HCC) is a major health problem with limited treatment options. The Sorafenib monothearpy is the only efficacious systemic treatment agent showing a survival benefit in these patie...
Effect of Ethanol Extract of Abrus precatorious Seed on Testosterone-Induced Benign Prostatic Hyperplasia in Adult Male Wistar Rats
Aim: To assess the effects of Abrus precatorious seed extract on prostate weight and hormonal levels (dihydrotestosterone, testosterone and estrogen) of male Wistarrats. Study Design: The study was divided into six group...
Circulating Prostate Gland Cells in Benign Prostatic Hyperplasia and Adenocarcinoma in Patients of African Heritage: Diagnosis by Liquid Biopsy: Preliminary Results
Background: Benign prostatic hyperplasia and adenocarcinoma are two major urological conditions of the human prostate gland. Benign prostatic hyperplasia is due to increase in stromal cells and adenocarcinoma of the pros...
Incidence of Multiple Mammary Tumors and Fibroadenoma in Pathological Study of Udder Affections in Camel (Camelus dromedarius)
Aims of the Study: To investigate the general histopathological pictures found in the udder tissue of camel (Camelus dromedarius) with emphasis on different types of tumors. Place and Duration of Study: The study was un...