Proliferative activity stem cells of culture bone marrow in dependence from cultural medium
Journal Title: Наукові горизонти. Scientific Horizons - Year 2018, Vol 66, Issue 3
Abstract
The article presents the results of research the influence composition culture medium on proliferative activity stem cells of bone marrow a cat. Animal experiments were conducted in compliance with the requirements of the law of Ukraine "On the protection of animals from cruelty" (2010). The stem cells bone marrow was obtained from aspirates of the contents cavity of femur bone a cat. To determine the optimal conditions of culture, cell precipitates were transferred into three different compositions culture medium. In order to determine the most effective of the following mediums, cells wereplaced in an amount of 400 thou. in cup (landing density 44 thou./ cm2) for each culture medium: 1. DMEM (Dulbecco's modified Eagle's medium) (D5648; SIGMA USA) and FBS (fetal bovine serum) - 20% with supplement antibiotic-antimycotic 10 μl / cm3; 2. DMEM (D5796; SIGMA USA) and FBS - 20% with supplement antibiotic-antimycotic 10 μl / cm3; 3. DMEM F12 (D8437; SIGMA USA) and FBS - 20% with supplement antibiotic-antimycotic 10 μl / cm3. Counting of the number of cells was made after 3 days of cultivation in a counting chamber Goryaev, after formation in one of the cups of the monolayer. The results of research were evaluated by the change the index of proliferation . The cell proliferation index at using DMEM (D5648) was 2.1, this confirmed doubling the cell mass from the time of sowing (the number of cells was 840 ± 23 thou.). When we using DMEM (D5796), the proliferation index for the third day was 1.43 (the number of cells was 570 ± 19 thou.). When used DMEM F12 (D8437), we were noted significant amount detachments of cell. After counting, were observed fewer adhesive cells compared with a landing number. The data received confirmed the proliferation index, which was 0.74. In comparative analysis of three culture media, we were found that the highest index of proliferation in the cultivation of stem cells bone marrow in the culture a cat was observed in a medium containing DMEM (D5648) in its composition.
Authors and Affiliations
V. Kovpak, O. Kovpak
Keywords
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- EP ID EP631604
- DOI 10.33249/2663-2144-2018-66-3-62-66
- Views 83
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