Propagation, Purification And Molecular Characterization Of Rvf Virus (Zh 501) Strain For Vaccine Production In Egypt
Journal Title: Journal of Applied Veterinary Sciences - Year 2019, Vol 4, Issue 1
Abstract
Rift Valley Fever (RVF) is an endemic disease in Egypt causing disease in animals and humans since 1977. Full molecular description of the national vaccine strain is a strategic concern to ensure the antigenic makeup of local vaccine is adequate to provide the required protective immunity for the vaccinated animal species and as a tool of final product quality control. Molecular characterization and phylogenetic analysis of the Gc gene of M segment of the RVF-ZH501 strain which used in local vaccine production in veterinary serum and vaccine research institute (VSVRI) were carried out in this study. RVF-ZH501 strain was propagated by passages on the BHK cell line and then purified by plaque purification technique. RT-PCR for amplification of the Gc gene of the M segment. Sequence analysis of the obtained PCR product carried out by the MEGA7 program. The result of the study revealed that RVF strain ZH501 Gc genomic content has 99.5 % molecular identity to the strains firstly isolated in Egypt in 1977, so still suitable for vaccine production.
Authors and Affiliations
Abo Hatab E. M. , M. H. Ali, Atwa M. H. , Abul Magd D. M. , Ahmed F. Soudy, Saad A. Moussa
Keywords
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- EP ID EP677359
- DOI 10.21608/JAVS.2019.62672
- Views 197
- Downloads 0
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