Protective effect of CSN1S2 protein of goat milk on ileum microstructure and inflmmation in rat-CFAinduced rheumatoid arthritis
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2015, Vol 5, Issue 7
Abstract
Objective: To observe the protective effect of goat milk alpha (S2)-casein (CSN1S2) protein on ileum microstructure and inflammation in rat-complete Freund’s adjuvant-induced rheumatoid arthritis model. Methods: Twenty four male Wistar rats were divided into six groups of two models. The body weight, food intake and albumin level of all subjects were calculated. The ileum microstructures were analyzed by scanning electron microscopy. Histopathological analysis was observed by hematoxylin-eosin staining and the level expressions of immunoglobulin E, secretory immunoglobulin A, interleukin-17, interleukin-10, Ki-67 and caspase-9 were measured by using western blotting. Results: CSN1S2 protein of milk or yogurt could repair the ileum villi of rat arthritis group similar to the normal. The level expressions showed the immunoglobulin E, secretory immunoglobulin A, interleukin-17 and caspase-9 decreased in milk CSN1S2 protein and yogurt CSN1S2 protein rat groups. The level expression of interleukin-10 was increased, and also Ki- 67 was significantly increased in milk CSN1S2 protein and yogurt CSN1S2 protein rat groups. CSN1S2 protein of milk and yogurt could increase the body weight and albumin significantly, meanwhile food intake increased but not significantly. Conclusions: CSN1S2 protein of goat milk and yogurt could repair the ileum microstructure, suppress inflammatory process and also increase the body weight, food intake and albumin level. This result indicates that goat CSN1S2 protein may protect the ileum disorder in rheumatoid arthritis disease.
Propolis-Sahara honeys preparation exhibits antibacterial and anti-biofilm activity against bacterial biofims formed on urinary catheters
Objective: To evaluate the antibacterial effect of Sahara honeys (SHs) against bacterial biofilms formed on urinary catheters in combination with propolis-Sahara honeys (P-SHs). Methods: Three clinical isolates were subj...
Absolute lymphocyte count: A useful surrogate marker to initiate anti retroviral therapy in resource poor settings.
Objective: Initiation of anti retroviral therapy (ART) is routinely based on the algorithms that combine CD4, HIV load and clinical illness. But their high cost and unavailability at resource poor settings are one of its...
Lessons learned from previous dengue outbreaks
Dengue is an important tropical infection caused by an arbovirus dengue. As a mosquito borne infection, this disease is widely spread in several tropical endemic countries. Millions of world populations are at risk for t...
Evaluation of anti-helmintic activity of Ferula foetida “Hing- A natural Indian spice” aqueous extract
Objective: To evaluate the anti-helmintic activity of Ferula foetida “Hing” against helmintic (Pheretima postuma). Methods: Aqueous extracts from the Ferula foetida resin was investigated for its anthelmintic activity ag...
Bilateral orbital abscesses with subdural empyema and cavernous sinus thrombosis due to melioidosis in a child
Orbital cellulitis is an emergency situation in children. Rapid diagnosis and appropriate management are mandatory to save both vision and life. In contrast, melioidosis infection causing orbital cellulitis with intracra...