Protective Effect of Either Dietary or Pharmaceutical n-3 Fatty Acids on Bone Loss in Ovariectomized Rats
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2010, Vol 3, Issue 1
Abstract
Background. Evidence suggests that n-3 polyunsaturated fatty acids (PUFA) are beneficial for maintenance of bone health and possibly bone development. Aim. To emphasize the effect of using either dietary flaxseed oil that was given as the sole source of fat in the diet, or orally given pharmaceutical omega-3 together with corn oil in preventing the development of osteoporosis among female surgically ovariectomized rats. Materials and Methods. Seventy two female white albino rats were used in this study, they all fed basal diet. They were divided into six groups: normal control (NC), sham (SH), ovariectomized (OVX), OVX received oral ethinyl estradiol (0.1 mg/kg bw/day), OVX fed with flaxseed oil (50 g/kg diet), OVX supplemented with Pharmaceutical omega-3 (30 μg/kg bw/day). All the groups were tested for bone turnover parameters (alkaline phosphatase, osteocalcin and insulin-like growth factor-1 for bone formation and deoxypyridinoline for bone resorption). Serum calcium and phosphorus were also determined and their excised femur bones were subjected for DEXA analysis and histopathological examination. Results. In OVX rats serum calcium was reduced significantly (p<0.01), while plasma osteocalcin, insulin-like growth factor-1, and urinary deoxypyridinoline were increased significantly (p<0.01) above those of sham and normal control. On either feeding with flaxseed oil or oral supplementation with pharmaceutical omega -3, the level of all these parameters were significantly changed towards normal (p<0.01). Both bone mineral content (BMC) and bone mineral density (BMD) were reduced in the OVX group, significant improvement was obtained after supplementation with flaxseed oil, omega- 3 or treatment with estradiol. Conclusion. Supplementation of n-3 fatty acids improved bone health and prevent osteoporosis in the ovariectomized animals.
Authors and Affiliations
Zakaria El-Khayat| Department of Medical Biochemistry, National Research Center (NRC), Tahreer Street Doki, Cairo 002, Egypt, Wafaa I. Rasheed| Department of Medical Biochemistry, National Research Center (NRC), Tahreer Street Doki, Cairo 002, Egypt, Tahany R. Elias| Department of Medical Biochemistry, National Research Center (NRC), Tahreer Street Doki, Cairo 002, Egypt, Jihan Hussein| Department of Medical Biochemistry, National Research Center (NRC), Tahreer Street Doki, Cairo 002, Egypt, Fatma Oraby| Department of Medical Biochemistry, National Research Center (NRC), Tahreer Street Doki, Cairo 002, Egypt, Manal Badawi| Pathology Department NRC, Cairo 002, Egypt, Soheir Salem| Department of Medical Biochemistry, National Research Center (NRC), Tahreer Street Doki, Cairo 002, Egypt
Keywords
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- EP ID EP8721
- DOI 10.3889/MJMS.1857-5773.2010.0080
- Views 324
- Downloads 20
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