Protective Effects of Lignite Extract Supplement on Intestinal Barrier Function in Glyphosate-Mediated Tight Junction Injury
Journal Title: Journal of Clinical Nutrition & Dietetics - Year 2017, Vol 3, Issue 1
Abstract
Background: Disruption of tight junctions is associated with various inflammatory diseases, including inflammatory bowel syndrome. Tight junctions form a protective barrier throughout the intestinal tract that is central to health. Glyphosate is known to inhibit tight junction function. The aim of this study was to assess the effect of a soil-derived, lignite extract dietary supplement in protecting against glyphosate’s effect on the tight junctions in cell culture. Methods: Small bowel (IEC-6) and colon epithelium (Caco-2) cells were incubated until a stable Transepithelial Electrical Resistance (TEER) was measured. A control and the lignite extract supplement (20% concentration) were placed on the cells and left overnight. These cells were then treated with and without glyphosate at 10 mg/ml. TEER was measured at 30 min. The expression of the tight junctions was determined by immunofluorescent microscopy of the ZO-1 tight junction element before and after overnight incubation, and following glyphosate exposure. Results: TEER increased with the lignite extract on IEC-6 (95%) and Caco-2 (35%) compared to the control. Glyphosate dramatically decreased the TEER in both IEC-6 (80%) and Caco-2 (76%) cells. The lignite extract blocked the glyphosatemediated decrease in TEER. Conclusion: The lignite extract supplement blocked a glyphosate-dependent decrease in TEER in both cell lines. If found efficacious in clinical trials, RESTORE could prove helpful in combating intestinal sensitivities caused by glyphosatemediated tight junction disruption.
Authors and Affiliations
John J Gildea, David A Roberts, Zachary Bush
Keywords
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- EP ID EP302402
- DOI 10.4172/2472-1921.100035
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