PROTEOLYTIC ACTIVITY OF PSEUDOMONAS CHLORORAPHIS 5N ISOLATED FROM RHIZOSPHERE SOIL
Journal Title: ELECTRONIC JOURNAL OF POLISH AGRICULTURAL UNIVERSITIES - Year 2012, Vol 15, Issue 4
Abstract
A Gram-negative bacterial strain characterized by high proteolytic activity was isolated from rhizosphere soil under winter wheat. Morphological and biochemical testing as well as analysis of 16S rRNA gene sequence identified the strain as Pseudomonas chlororaphis. In this study an extracellular endopeptidase synthesized by the strain was purified and characterized. The enzyme showed highest activity at pH 11 and at temperature 42°C. The molecular weight determined by SDS-PAGE was approx. 51 kDa. The studied endopeptidase was characterized by low thermal stability in the 37-45°C range. The activity of the enzyme was strongly inhibited by specific metallopeptidase inhibitors, which allowed classifying the studied protein as a metalloendopeptidase. Ca2+ and Mg2+ ions stimulated the activity of the enzyme, whereas Mn2+, Co2+ and Cu2+ ions inhibited it.
Authors and Affiliations
Urszula Jankiewicz, Magdalena Frąk
Keywords
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