Protoplast as a tool to address questions in plant physiology
Journal Title: Journal of Plant Development - Year 2018, Vol 25, Issue
Abstract
A protoplast is a plant cell from which the cell wall has been removed by enzyme treatment. Tremendous utilization of protoplast in molecular analysis of plant growth and development has been observed in the past decades and the system has paved its way to significantly facilitate the comprehensive understanding of the complexity of underlying mechanisms. However, it should be kept in mind that a plant, like all systems, is composed of networks of interdependent components that integrate the system into a unified whole. In this mini review, we will re-explore protoplast approach in answering plant physiology questions through discussion of its application in the study of (1) photosynthesis and chloroplast-related process; (2) pollen tube growth; (3) sieve tube element protoplast for long distance translocation; (4) new regulatory metabolites from guard cell protoplast. This in vitro approach may open the way to further meaningful results at organismal level.
Authors and Affiliations
XING Tim, TIAN Lining
Keywords
Related Articles
- EP ID EP454704
- DOI 10.33628/jpd.2018.25.1.99
- Views 73
- Downloads 0
Studies on epidermal appendages from vegetative organs at Euphorbias pecies cultivated in Botanical Garden Iassy
The Euphorbia genus is unitary by the presence of the characteristic inflorescence named cyathium, but presents a remarkable morphological diversity of aerial vegetative organs. Species that originated in the tropical zo...
Spatial distribution of some ectomycorrhizal fungi (Russulaceae, Fungi, Basidiomycota) in forest habitats from the North-Eastern Region (Romania)
Ectomycorrhizal macromycetes are, generally, an important ecological component for forest habitats, and a valuable resource in the context of sustainable development of rural communities in the North-East Region of Roman...
Volatile compounds in the aroma of three species of wood-rotting basidiomycetes and their antifungal potential
rotting basidiomycetes: Coriolopsis gallica, Megacollybia platyphylla and Lentinus arcularius and test their antifungal potential. The species were cultivated on liquid media and kept for 25 days at 25 °C. The surface cu...
Knockout of AtMKK1 reduces Arabidopsis response to 2, 3, 5-triiodobenzoic acid in leaves
Mitogen activated protein kinase (MAPK) pathways are crucial for plant growth and development. The most commonly identified pathways that AtMKK1 has been connected to are wounding, bacterial pathogen response, cold, drou...
Dispersal of Rafflesia patma Blume endophyte in grafted host plant (Tetrastigma leucostaphylum (Dennst.) Alston).
This study provides a hypothetical discussion about the growth of Rafflesia patma Blume (syn. R. horsfieldii R. Br. (1821); Rafflesiaceae), an endophytic parasite, within a grafted host, a woody vine (Tetrastigma leucost...