PSEUDOMONAS- AN EMERGING NOSOCOMIAL PATHOGEN
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2019, Vol 8, Issue 1
Abstract
BACKGROUND Pseudomonas aeruginosa is one of the most common pathogens causing nosocomial infections, which is a major threat to patients’ safety. It is an opportunistic pathogen with innate resistance to many antibiotic classes. Resistance to carbapenems is often mediated by metallo-betalactamase production. It also expresses many extracellular virulence factors. The rising incidence of nosocomial infection stress the need to detect the pathogenic mechanism. Aims and Objectives1. To identify and speciate Pseudomonas from various clinical isolates2. To detect antimicrobial susceptibility pattern of Pseudomonas. 3. To detect metallo-betalactamase (MBL) production in Pseudomonas. 4. To detect virulence factors in Pseudomonas (haemolysin, gelatinase, deoxyribonuclease, bile aesculin, biofilm). MATERIALS AND METHODS 150 samples were processed. Isolation, identification and speciation of the samples were done by conventional biochemical tests and antimicrobial susceptibility testing using CLSI 2017 guidelines by Kirby Bauer disc diffusion method. Metallo-Betalactamase production was detected by combined disc diffusion test and E strip method. Virulence factors were detected by inoculating and incubating them on blood agar, gelatin agar, bile esculin agar, DNase test agar and biofilm by microtitre plate method. Statistical Analysis- p value was determined by Chi square test using SPSS software Setting and Design- It was a Descriptive Study conducted between December 2016 and May 2018 in the Department of Microbiology at a tertiary care hospital. RESULTS Pseudomonas aeruginosa (50%) was the major isolate. Antimicrobial susceptibility was maximum for meropenem (59%) and amikacin (59%). Metallo-betalactamase production was detected in 60 isolates by combined disc diffusion method and it was confirmed by E strip method which detected metallo-betalactamase production in 56 isolates. Maximum virulence factor produced was haemolysin (100%). CONCLUSION There has been a rising incidence of Pseudomonas nosocomial infection with resistance to multiple groups of drugs which is a serious threat. Preventive measures should be taken to prevent the spread of infection.
Authors and Affiliations
Tushar Gautam, Anjana Gopi
Keywords
Related Articles
- EP ID EP553006
- DOI 10.14260/jemds/2019/2
- Views 98
- Downloads 0
A CASE OF IDIOPATHIC PULMONARY ARTERIAL HYPERTENSION IN MALE
Primary Pulmonary Hypertension is a rare disease occurring in 1-2 per million population. It is 2-4 times more common in female. Idiopathic or primary pulmonary hypertension is defined as a disorder with no ident...
INTUSSUSCEPTION IN ADULT MALE
BACKGROUND Intussusception is highly uncommon in adults and accounts for 5% of all reported cases, Dean DL et al[1] and Gayer G, and Apter S[2] compared to children, where it is more often encountered. A definite identif...
HETEROTOPIA
: common causes of epilepsy with the advent of modern high-resolution MR scanning. Among these, heterotopias are the most common developmental disorders in patients with epilepsy.1 Heterotopia is the name give...
RANDOMIZED CONTROL TRIAL TO EVALUATE THE ROLE OF DEXMEDETOMIDINE PREMEDICATION & KETAMINE- PROPOFOL COMBINATION FOR ATTENUATION OF POST ECT DEPRESSION AND AGITATION
BACKGROUND Electroconvulsive Therapy is associated with hyperdynamic response which can lead to cardiac dysrhythmias, myocardial ischemia or infarction. Dexmedetomidine is alpha-2 agonist with anxiolytic, sedative, sympa...
MANAGEMENT STRATEGIES IN A CASE OF POTT`S SPINE WITH DOWN SYNDROME- A RARE ENTITY
PRESENTATION OF CASE A 14 yrs. old male child, a known case of Down syndrome was referred to our center with chief complaints of gradual onset pain in mid-back region and inability to walk for the past 5 months. He also...