Purification and Characterization of Cellulase enzyme from Trichoderma longibrachiatum isolated in Iraqi soil
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 1
Abstract
Cellulase produced by Trichoderma longibrachiatum was purified throughout four purification steps includes ammonium sulfate precipitation step with a saturation ratio of 80% then dialysis step followed by purification with ion exchange chromatography by using DEAE-cellulose, and then gel filtration step throughout sephadex-G200 which allows larger ability of separation with high degree of purification. Results of purification showed that the specific activity of the purified enzyme was 6680.8 U/mg protein with a purification fold and yield of 9.69 and 68.8% respectively. Purified cellulase from Trichoderma longibrachiatum was well characterized by studying some enzyme characteristics. Results showed that the molecular weight of cellulase was 17782 kilodalton; pH 4 was the optimum for enzyme activity and stability, while 40 ºC was also the optimum for enzyme activity and stability.
Authors and Affiliations
Noor. T. Hamdan, Hameed M. Jasim
Keywords
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- DOI 10.9790/264X-04013241.
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