Purification and Structure Elucidation of the Polyether Antibiotic Alborixin from Streptomyces pulcher CRF17
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 6
Abstract
Aims: To screen Streptomyces sp. from saline soil in Pakistan, for its antimicrobial activity and to purify and identify the active metabolites produced by mass spectrometry and NMR spectroscopy. Strain identification by morphological, biochemical, physiological characterization and by 16S rRNA gene sequencing. Study Design: Cultivation in lab fermenter, solvent extraction and purification of the compounds by column chromatography, identification of the compounds by mass spectrometry and NMR spectroscopy, determination of the antimicrobial activity and cytotoxicity. Place and Duration of Study: Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan and Institute of Organic and Bio molecular Chemistry, University of Göttingen, Germany, between February, 2007 and April, 2009. Methodology: The strain was cultivated in a 20 liter fermenter (working volume 10 liters) and the culture broth was extracted with ethyl acetate, acetone and methanol. The resultant crude extract was fractionated on silica gel and the components were purified by column chromatography (silica gel, sephadex column and preparative TLC). The pure component was identified by mass spectrometry (ESI and HRESI-MS), NMR analysis (1H and 13C NMR) and by comparison with reference data, the antimicrobial activity and cytotoxicity was determined by disc diffusion assay and by micro well cytotoxicity assay against Artimia salina. Results: The morphological, biochemical and physiological characterization suggested that isolate CRF17 belongs to the genus Streptomyces. Further NCBI BLAST of the partial 16S rDNA gene sequence 1420 bp (gene bank accession number: EU294134) from the isolate CRF17 showed 99% identity and 98% query coverage towards Streptomyces pulcher. The scale up fermentation of the isolate CRF17 yielded active compound and was identified as alborixin (1). Conclusion: The isolate Streptomyces pulcher CRF17 is a potent producer of the antibiotic alborexin and can be exploited for its commercial production.
Authors and Affiliations
Imran Sajid, Khaled A. Shaaban, Shahida Hasnain
Keywords
Related Articles
- EP ID EP344841
- DOI 10.9734/BJPR/2014/5721
- Views 96
- Downloads 0
Heat Stable Inhibitors of Protein Cross-linking from Sri Lankan Medicinal Plants
Aims: Glycation induced protein cross-linking is recognized as the most damaging element of advanced glycation end product formation and is a key player of diabetic complications. Objectives of the present study were to...
Phytochemical and Pharmacological Profiles of the Genus Odontonema (Acanthaceae)
Odontonema is a group of tropical plant species used in folklore medicine because of its wide range of pharmacological properties. These plants are known to be anti-bacterial, anti-inflammatory, anti-hypertensive, anti-v...
A Novel Acyl-CoA: Diacylglycerol Acyltransferase 1 (DGAT1) Inhibitor, GSK2973980A, Inhibits Postprandial Triglycerides and Reduces Body Weight in a Rodent Diet-induced Obesity Model
Background and Aim: Acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) is a key enzyme catalyzing the final step of triglyceride (TG) synthesis and is implicated in fat absorption and disposition. However, development of...
Molecular Detection of Mycobacterium tuberculosis Complex by GenoType®MTBDRplus from Patients Attending Bingham University Teaching Hospital, Jos, Nigeria
Aims: The study was to investigate the presence of M. tuberculosis and the resistance to rifampicin and isoniazid in AFB-positive sputa of patients attending Bingham University Teaching Hospital, Jos, Nigeria. Study Desi...
Potential Antioxidant and Anti-Proliferative Activities of Biologically Active Marine Algae Extracts
Background: Marine microorganisms such as algae were identified a rich sources of biologically active compounds. This study was designed to explore the in vitro antioxidant, anti-apoptotic and anti proliferative activiti...