QUANTIFICATION BIAS IN BLOOD ALCOHOL DETERMINATION BY HEADSPACE GAS CHROMATOGRAPHY
Journal Title: International Journal of Medical Laboratory Research - Year 2019, Vol 4, Issue 1
Abstract
The quantification of blood alcohol concentration is routinely performed in many forensic laboratories by gas chromatography. In this short report, the influence of the matrix miss-match between samples and calibrators (e.g. the use of aqueous standards to quantify blood samples) and the actual efficiencies of different internal standards used to normalise the ethanol signal were considered. Test samples in blood and water were prepared from a certified reference standard of ethanol. The samples were analysed by gas chromatography using n-propanol and t-butanol as internal standards. The collected data provided evidences that a sub-optimal setting of the considered variables can be responsible for a quantification bias up to 15%. These issues are of practical relevance in the medico-legal sector and should be considered during the process of method development.
ASSESSMENT OF CYTOLOGY GRADING AND CLINICAL PARAMETERS IN PREDICTING THE EXTENT OF DAMAGE TO THE THYROID GLAND IN CASES OF CHRONIC LYMPHOCYTIC THYROIDITIS: A PROSPECTIVE STUDY
Background: Chronic lymphocytic thyroiditis presents with a highly variable range of presentation and varying degrees of damage to the thyroid at the time of diagnosis. For most of the patients, duration of symptoms is t...
ANTIBIOTIC RESISTANCE PATTERN OF GRAM NEGATIVE BACTERIAL ISOLATES FROM THE CLINICAL SAMPLES FROM CRITICAL CARE UNITS OF A TERTIARY CARE CENTRE
Antimicrobial resistance (AMR) is a major issue especially in patients admitted in Intensive care units. Irrational overuse and misuse of antibacterial drugs has made the pathogens resistant to them. In eastern countries...
IMMUNOHISTOCHEMICAL CORRELATION BETWEEN THE EXPRESSION OF VITAMIN D RECEPTOR (VDR) AND ESTROGEN RECEPTOR (ER) IN INVASIVE DUCTAL CARCINOMA TISSUES
Aim: The study was carried out to determine the Immunohistochemical Correlation between the Expression of Vitamin D Receptor (VDR) and Estrogen Receptor (ER) in Invasive Ductal Carcinoma (IDC) tissues Materials and Metho...
ACUTE PROMYELOCYTIC LEUKEMIA (APL): DIAGNOSIS AND TREATMENT
Background:Acute Promyelocytic Leukemia (APL) is a subgroup of Acute Myeloid Leukemia(AML) with a different certain clinical period, biology and treatment. APL was the deadly leukemia but today it is most curable leukemi...
TYPES OF PRE-ANALYTICAL ERRORS IN CLINICAL HEMATOLOGY LABORATORY
Background: Since, clinical laboratory results play an important role in patient’s diagnosis, investigation and treatment. Thus, errors with laboratory tests might lead to inappropriate medical decision-making. Such erro...