Quantitation of TLR-2 mRNA Expression in Bovine Mastitis Caused by E. coli
Journal Title: International Journal of Biochemistry Research & Review - Year 2017, Vol 17, Issue 3
Abstract
Aims: This study aim was to assay the expression of an important TLR2 gene in naturally infected cows with sub-clinical and clinical mastitis caused by E. coli. Methodology: cDNA from E. coli infected samples were obtained from hind quarters of three animals with sub-clinical mastitis, three animals with clinical mastitis along with a healthy animal, were used for TLR2 expression analysis using commercial kit and oligo (dT) primers. Quantitative reverse transcription PCR (RT-qPCR) were standardized for the TLR2 and β-actin gene, genes expression level was analyzed by Illumina Eco® Q- RT PCR system. Results: The mRNA expression of TLR2 gene in animals sub-clinical mastitis was significant (P ≤ 0.01) higher (3.63 fold) than clinical case (1.89 fold) when compared to healthy bovine. Conclusion: The expression of TLR2 gene during early stage of infection was at high level, therefore most of the sub-clinical mastitis subsided by itself without precipitating into clinical mastitis. It is suggested that TLR2 may consider as candidate gene for mastitis screening in cattle population.
Authors and Affiliations
R. Lakshmi, K. K. Jayavardhanan
Keywords
Related Articles
- EP ID EP317073
- DOI 10.9734/IJBCRR/2017/33367
- Views 121
- Downloads 0
Hepatic Involvement with Elevated Liver Enzymes in Nepalese Subjects with Type 2 Diabetes Mellitus
Background: Diabetes mellitus (DM) is characterized by a broad disturbance in metabolism. Since the liver plays a vital role in the regulation of metabolism, there is evidence of liver dysfunction in DM. There is a high...
Effect of Enzyme Replacement Therapy on Disease Burden Biomarkers in Gaucher's Disease
Aims: We evaluate the effect of enzyme replacement therapy "ERT" on the plasma levels of some biochemical markers of the disease burden, in pediatric patients with Gaucher's disease "GD", in the form of plasma chitotrios...
Triterpenes and Triterpenoids Clinically Useful with Multiple Targets in Cancer, Malaria and More Treatment: Focus on Potential Therapeutic Value
Triterpenoids are the most promising plant secondary metabolites. Several triterpenoids, including ursolic acid, oleanolic acid, betulinic acid, celastrol, pristimerin, lupeol and avicins are representative group of phyt...
A Novel Approach to Detect Cellular Signals of Oxidative Stress Due to Extreme Environmental Temperatures in Backyard Broiler from Arid Tracts in India
The present investigation was aimed to investigate use of novel methods to detect cellular signals of oxidative stress due to extreme environmental temperature periods (ETPs) in broilers from arid tracts in India. ETPs i...
The Effect of Aqueous Extract of Buchholzia coriacea Seeds on Some Biochemical Parameters in Normal and Alloxan-induced Diabetic Rats
Aims: To investigate the effect of oral administration of Buchholzia coriacea aqueous seed extract on blood glucose and other biochemical parameters in alloxan-induced diabetic rats and in normal rats. Study Design: Twe...