Quantitative reckoning of embelin from fruits of Embelia tsjeriam- cottam using water bath process as an alternate method of extraction
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2015, Vol 3, Issue 3
Abstract
The world is culturally endowed with various forms of healing practices having rich medical wisdom of immense importance. One of such pharmacognostically indispensable medicinal plant is Embelia tsjeriam-cottam A. DC. The active principle of E. tsjeriam-cottam is embelin, possessing a range of pharmacognostic activities including anti-cancer, antioxidant, antiinflammation, antibacterial and analgesic effects. Embelin in fruits of E tsjeriam-cottam was extracted using water bath method. For extraction of embelin from fruits of E. tsjeriamcottam, methanol and chloroform were used as solvents. Fruits were collected from five different agro-climatic zones of Odisha. Comparative estimation of embelin was done through spectrophotometer and High Performance Liquid Chromatographic (HPLC) methods. Samples extracted with chloroform and methanol showed embelin content in a range of 2.13- 0.29% dry wt., and 0.95-0.28% dry wt. respectively using spectrophotometer. In case of HPLC analysis, samples extracted in chloroform and methanol showed embelin content in a range of 1.86-0.27% dry wt., and 0.875-0.26% dry wt. respectively. However the water bath method, used as alternative method for extraction, proved to be less time consuming, costeffective in extracting a pretty good amount of embelin as compared to the conventional (soxhlet) methods of extraction.
Authors and Affiliations
M Mohapatra| Seed Bank & Seed Biology Division, Regional Plant Resource Centre, R & D Institute of Forest and Environment Department, Govt. of Odisha, Bhubaneswar, Odisha, India, U C Basak| Seed Bank & Seed Biology Division, Regional Plant Resource Centre, R & D Institute of Forest and Environment Department, Govt. of Odisha, Bhubaneswar, Odisha, India
Keywords
Related Articles
- EP ID EP15342
- DOI -
- Views 309
- Downloads 13
Small-scale screening program for the identification of cytotoxic Oxazolo[5,4-d]pyrimidine derivatives based on Whole Cell Viability Assay
For over last couple of decades, there has been a robust activity aimed towards the discovery of novel anti-cancer therapeutics. An approach to identify starting points for new drug candidates is high throughput screen...
Formulation and Dissolution Study of Valsartan Immediate Release Tablets
In the present study, design of oral immediate release tablets of Valsartan by direct compression technique was carried out. The main aim and objective of the work is to formulate immediate release tablets using differ...
Melatonin and thermal stress regulate differential expression of heat shock proteins and melatonin receptors in spleen of mice
Cellular defence by expression of heat shock proteins (Hsps) against stress stimulation is the most universal phenomenon in stress physiology. Melatonin is well known as an anti-stress molecule possessing hypothermic e...
Capparidaceous Medicinal Plants of North-West Rajasthan: Good Sources of Ascorbic Acid
Ascorbic acid contents of roots, shoots and fruits of three selected medicinal plants like Capparis decidua, Cleome gynandra and Cleome viscosa of family Capparidaceae have been analysed. Maximum ascorbic acid contents...
Morphological responses of a high yielding groundnut cultivar (Arachis hypogaea L. cv. K-134 ) under water stress
The present study was undertaken to ascertain the morphological responses of a groundnut cultivar K-134, under different water stress regimes characterized as control, mild, moderate and severe stress represented by 100,...