Radical Scavenging Capacity and Efficacy of Myristica fragrans (Nutmeg) Metabolites on Cladosporum herbarum of Food Origin
Journal Title: Microbiology Research Journal International - Year 2017, Vol 20, Issue 1
Abstract
Aim: To determine radical scavenging capacity and efficacy of Myristica fragrans metabolites on fungi of food origin. Study of Design: Pretreatment and processing of Myristica fragrans, solvent extraction technique, phytochemical screening, radical scavenging activity, total phenolic concentration assay, essential oil extraction and evaluation of antifungal activity. Place and Duration of Study: Microbiology Unit, Department of Biological Sciences, College of Natural and Applied Sciences, Fountain University Osogbo, Osun State, Nigeria between October, 2015 and July, 2016. Methodology: Crude extract of Myristica fragrans seed was obtained using organic solvent (distilled water, ethyl acetate and ethanol) with solvent extraction technique and preliminary phytoconstituents was determined. The essential oil was extracted by hydrodistillation technique and isolated with petroleum ether. Metabolites present in the essential oil were quantified using Gas Chromatography Flame Ionization Detection (GCFID). Antifungal activity of Myristica fragrans oil and crude extract were investigated using agar well diffusion method. Folin-Ciocalteu and 2,2, diphenyl picryl hydrazyl (DPPH) radical scavenging assays were employed to determine total phenolic content and antioxidant activity respectively. Results: Preliminary phytochemical screening of Myristica fragrans seed revealed the existence of alkaloid, phenols, flavonoids, terpenes, saponins, glycosides, tannins, steroids and phenolic compounds. The percentage yield of Myristica fragrans oil extracted was 3.25%. Thirty-six metabolites were quantified in Myristica fragrans essential oil using GCFID among which are sabinene (26.58%), myristicin (13.55%), alpha-pinene (11.84%), terpinene-4-ol (9.35%), limonene (5.74%), safrole (5.40%), alpha-terpineol (4.51%), alpha-myrcena (3.82%), gama- terpinene (3.71%), alpha-terpinolene (3.19%), pinene-2-ol (1.84%), elimicin (1.27%) and isoeugenol (1.13%) respectively. Highest scavenging and antifungal activities were observed in ethyl acetate extract of Myristica fragrans compared to Beta-carotene and antifungal drug (Fluconazole) used as control at varying concentrations. Conclusion: Presence of thirty-six different phytoconstituents (metabolites) in Myristica fragrans essential oil poses the potential of providing useful drugs for treating food-borne infection and reduction of oxidative stress in the body other than its general uses as spices and flavoring agent.
Authors and Affiliations
Simiat Olanike Jimoh, Olaoniye Habeebat Labo-Popoola, Kazeem Adelani Alabi
Keywords
Related Articles
- EP ID EP315962
- DOI 10.9734/MRJI/2017/31962
- Views 103
- Downloads 0
Characterization of Gordonia sinesedis Isolated from a Zebu Cow Suffering from Lymphadenitis
Most of the Gordonia species described earlier have been considered as opportunistic pathogens in humans, but recently described species have been isolated from the environment with notable roles in bioremediation or the...
Detection of Sutterella in the Stool of Egyptian Children with Autism Spectrum Disorders
Background: Autism spectrum disorder (ASD) is a multifaceted group of neurodevelopmental disorders. Gastrointestinal problems are commonly reported in children with autism and may correlate with autism severity. A recent...
In vitro Assessment of the Antibacterial Activity of Matricaria chamomile Alcoholic Extract against Pathogenic Bacterial Strains
An alarming increasing in the occurrences of antimicrobial resistance inside the existing clinical use and so the recent appearance of multidrug resistant bacteria that attempts the treatment of infections necessities to...
Degradation of Polycyclic Aromatic Hydrocarbons and Aroma Oxidation of Fermented Locust Bean Seeds ‘Iru’ by Laccase Enzyme Produced by Kluyveromyces dobzhanskii DW1 and Pichia manshurica DW2
Laccases (EC.1.10.3.2 are multi-phenolics and non-phenolic oxidizing enzymes) have received much attention in their use for environmental bioremediation, food quality and functionality improvement. This work was aimed at...
The Antibacterial Effect of Carica papaya L. Extracts and Their Synergistic Effect with Antibiotic and Non-antibiotic Drugs
Aims: Antibacterial activity of Carica papaya leaf and seed extracts, their synergism with antibiotic and non-antibiotic drugs and GC-MS analysis of extracts. Study Design: Antibacterial activity was evaluated by Disc an...